Biodegradation of Residual Petrochemicals and Microbial Activities in Polluted Soil
-
摘要: 利用色谱-质谱联机(GC/MS)、柱层析分析方法、浊度法和微生物脱氢酶法, 确定土壤残油污染物的构成、油污土壤优势降解菌、残油降解过程中微生物活性及其动态变化.研究结果表明, 长期残留在淄河滩土层中的石油烃类主要由沥青质、胶质构成, 占总烃类的6 0 %~80 %.经过90d生物降解试验, 残油降解率仅为2 0 %.污染土层中的主要优势菌为球菌和杆菌, 菌群鉴定为黄假单胞菌(Xanthonmonas)、芽孢杆菌(Bacillus) 等.据微生物脱氢酶动态变化的初步研究结果, 微生物脱氢酶活性变化可作为衡量微生物活性变化的重要指标.Abstract: The components of the residual petrochemicals, variation of the microbe dehydrogenase activities and screening hydrocarbon degrading bacteria in the polluted soil of Zihe valley were measured by GC/MS, column chromatography, turbidity and microbe dehydrogenase methods. The experimental results indicate that petroleum carbons in the soil are composed of colloid and pitchy compounds accounting for 60%~80% of total petroleum carbons, which may limit the biodegradation of petrochemicals. It was proved that after 90 days the biodegradation rate of the petrochemicals was only 20%. The major types of the degrading bacteria in the polluted soil are cocci, or short and long bacilli based on the degradation experiments of naphthalene and liquid paraffin. Xanthomonas, Bacillus and a species thought to be Hyphomicrobium are identified to have high activity in the experiments. The pilot studies present that the microbe dehydrogenase can be used as index of the microbe activities for the biodegradation of petrochemicals.
-
Key words:
- residual petrochemicals /
- biodegradation /
- microbial dehydrogenase.
-
图 2 优势降解菌电镜扫描照片
Fig. 2. Electron microscope pictures of degrading bacteria in polluted soil
表 1 油污土壤石油烃Soxhlet抽提、柱层析分析结果
Table 1. Components of residual petroleum in polluted soil by Soxhlet extraction and column-chromatography
表 2 除油菌数量的动态变化
Table 2. Variations of degrading bacterium cells
-
[1] 祁士华, 盛国英, 傅家谟, 等. 鼎湖山自然保护区大气溶胶中的PAHs[J]. 地球科学——中国地质大学学报, 2001, 26(1): 83-87. https://www.cnki.com.cn/Article/CJFDTOTAL-DQKX200101015.htmQI S H, SHENG G Y, FU J M, et al. PAHs in aerosols at Dinghushan natural protection zone[J]. Earth Science -Journal of China University of Geosciences, 2001, 26 (1): 83-87. https://www.cnki.com.cn/Article/CJFDTOTAL-DQKX200101015.htm [2] 中华人民共和国石油天然气行业标准. 岩石可溶有机物和原油族组分柱层析分析方法, S/Y5119[S]. 中国石油天然气总公司, 1995.Standards of oil and gas industry of People's Republic of China. Analysis method of column chromatography of soluble organic compounds and crude oil family composition in rock. S/Y5119[S]. Chinese National Petroleum Cooperation, 1995. [3] 李东艳, 钟佐, 孔惠, 等. 反硝化条件下苯生物降解的微环境研究[J]. 地球科学——中国地质大学学报, 2000, 25(5): 493-498. https://www.cnki.com.cn/Article/CJFDTOTAL-DQKX200005010.htmLI D Y, ZHONGZ S, KONG H, et al. Microcosm study of benzene degradation on nitrate reducing conditions[J]. Earth Science-Journal of China University of Geosciences, 2000, 25(5): 493-498. https://www.cnki.com.cn/Article/CJFDTOTAL-DQKX200005010.htm [4] 郭华明, 王焰新, 陈艳玲, 等. 地下水有机污染的水文地球化学标志物探讨[J]. 地球科学——中国地质大学学报, 2001, 26(3): 304-308. https://www.cnki.com.cn/Article/CJFDTOTAL-DQKX200103013.htmGUO H M, WANG Y X, CHEN Y L, et al. Geochemical indicators of organic contamination in groundwater: a case study in Henan oilfield[J]. Earth Science-Journal of China University of Geosciences, 2001, 26 (3): 304 - 308. https://www.cnki.com.cn/Article/CJFDTOTAL-DQKX200103013.htm [5] Randall J D, Hemmingsen R B. Evaluation of mineral Agar plates for the enumeration of hydrocarbon-degrading bacteria[J]. Journal of Microbiologica Methods, 1994, 20: 103-113. doi: 10.1016/0167-7012(94)90013-2 [6] Schnurer J, Rosswall T. Flourescein diacetate hydrolysis as ameasure of total microbial activity in soil and litter[J]. Applied and Environmental Microbiology, 1982.1256 - 1261. [7] Federle T W, Dobbins D C, Thornton-Manning J T, et al. Activity and community structure in subsurface soils [J]. Groundwater, 1986, 24(3): 534-543. [8] 郑西来, 邱汉学, 荆静, 等. 沈抚灌区石油污染土壤恢复方案的数值模拟[J]. 地球科学——中国地质大学学报, 2000, 25(5): 462-466. https://www.cnki.com.cn/Article/CJFDTOTAL-DQKX200005003.htmZHENG X L, QIU H X, JING J, et al. Numerical modeling on elimination of oil-polluted soils in Shenyang-Fushun irrigation area[J]. Earth Science-Journal of China University of Geosciences, 2000, 25(5): 462-466. https://www.cnki.com.cn/Article/CJFDTOTAL-DQKX200005003.htm [9] McGugan B R, Lees Z M, Senior E. Bioremediation of an oil-contaminated soil by fungal intervention[A]. In: Hinchee R E, Vogal C M, Brockman F J, eds. Microbial process for bioremediation[C]. New York: Battelle Press, 1996.149-156. [10] Mahro B, Schaefer S, Kastner M. Pathways of microbial degradation of polycyclic aromatic hydrocarbons in soil [A]. In: Hinchee R E, VogalC M, BrockmanF J, eds. Bioremediation of chlorinated and PAH compound[C]. New York: Battelle Press, 1996.203-217. -
下载:
点击查看大图
图(3) / 表(2)
计量
- 文章访问数: 3669
- HTML全文浏览量: 444
- PDF下载量: 7
- 被引次数: 0
