高效纤维素降解菌系的构建

李平; 王焰新; 刘琨; 王艳红; 童蕾

高效纤维素降解菌系的构建

中国地质大学环境学院, 生物地质与环境地质教育部重点实验室, 湖北武汉 430074

基金项目:

国家杰出青年科学基金 40425001

世界自然基金会资助课题 CN0879.01-2.5.02.01

详细信息

作者简介:
李平(1975-), 女, 博士, 主要从事环境生物、污染控制方面的研究.E-mail: plicug@gmail.com

中图分类号: X17
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出版历程
- 收稿日期: 2009-01-12
- 刊出日期: 2009-05-25

Construction of A Microbial System for Efficient Degradation of Cellulose

School of Environmental Studies, MOE Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China

摘要

摘要: 筛选出能产生不同纤维素酶的10株纤维素降解菌, 系统地分析了各菌株的EG、CBH和BG酶等3类纤维素酶活.经各菌株优化组合、混合培养, 构建了一组由5株细菌(LCB03、LCB12、LCB52、LCD12和LCD51)组成、能协同作用的复合微生物菌系.经生理生化和分子水平鉴定, 这5株细菌分别为Pseudomonas citronellolis(香茅醇假单胞菌)、Stenotrophomonas malto-philia(嗜麦芽寡食单胞菌)、Pseudomonas aeruginosa(铜绿假单胞菌)、Pseudomonas aeruginosa(铜绿假单胞菌)和Flavobacterium mizutaii(水氏黄杆菌).复合菌系的各菌株可产生不同类型的纤维素酶, 且各类酶可以协同作用有效分解天然纤维素, 在纤维素类污染的治理与资源化利用中具有很好的应用前景.
- 纤维素降解 /
- 复合菌系 /
- 构建 /
- 鉴定
Abstract: Endoglucanase, exoglucanase and cellobiase activities of ten cellulose degradation isolates from piggery sludge and manure were analyzed.A complex microbial system was constructed by five bacteria named as LCB03, LCB12, LCB52, LCD12 and LCD51 respectively with different cellobiase activities from these isolates.Tested from the perspective of physiology and microchemistry as well as molecular level, these five bacteria were identified as Pseudomonas citronellolis, Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Pseudomonas aeruginosa and Flavobacterium mizutaii, respectively.So far, there are few papers on cellulose degradation using S.maltophilia and F.mizutaii.Each of the five bacteria could produce different types of cellobiase that co-catalyze degrading natural cellulose effectively, and the microbial system therefore has great potential in cellulose-related pollution control and waste utilization.
- cellulose biodegradation /
- complex microbial system /
- construction /
- identification

HTML全文

图 1 各菌株的3种纤维素酶活

Fig. 1. Endoglucanase, exoglucanase and cellobiase activities of the ten isolates

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图 2 复合菌系降解天然纤维素稻草的各种酶活

Fig. 2. Endoglucanase, exoglucanase and cellobiase activities of degrading natural cellulose by composite microbial system

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图 3 5株细菌16S rDNA的PCR产物琼脂糖电泳

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图 4 菌株LCB03、LCB12、LCB52、LCD12和LCD51基于16S rDNA序列的系统发育树

Fig. 4. Phylogenetic tree generated from an alignment of the 16S rDNA of strain LCB03, LCB12, LCB52, LCD12 and LCD51

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表 1 刚果红鉴别培养基透明圈直径(mm)

Table 1. Degrading capability identification by Congo

表 2 平板混合培养情况

Table 2. Growth of bacteria on mixed culture plates

表 3 五株细菌主要的生理生化特征

Table 3. Main physiological and biochemical characteristics of five isolates

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