土著微生物参与下河套平原地下水中砷的还原作用

杨会; 王焰新; 谢先军; 段萌语

doi:10.3799/dqkx.2011.061

土著微生物参与下河套平原地下水中砷的还原作用

doi: 10.3799/dqkx.2011.061

杨会^{1, 2,},
王焰新²,
谢先军²,
段萌语²

1.
中国地质科学院岩溶地质研究所, 广西桂林 541004
2.
中国地质大学环境学院, 教育部生物地质与环境地质重点实验室, 湖北武汉 430074

基金项目:

国家杰出青年科学基金项目 40425001

国家自然科学基金重点项目 40830748

详细信息

作者简介:
杨会(1982-), 女, 硕士研究生, 研究方向为环境地球化学.E-mail: hy53022@163.com

中图分类号: P593
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- 被引次数: 0
出版历程
- 收稿日期: 2010-07-29
- 刊出日期: 2011-05-01

Reduction of Arsenic in Groundwater from Hetao Plain with the Involvement of Indigenous Microbes

1.
Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, China
2.
School of Environmental Studies, MOE Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China

摘要

摘要: 为查明土著微生物活动对高砷地下水形成的影响,利用河套平原高砷地下水中分离出的土著微生物(YH002)进行了微宇宙实验研究.实验结果表明: 高砷地下水中加入的葡萄糖提供了微生物生长所需要的碳源,微生物大量繁殖,分泌的有机酸使溶液的pH值降低.在缺氧条件下,溶液中的OD值最高达到了0.189,pH值最低为6.22;在有氧条件下,OD值最高达到了0.286,pH值最低为6.04.溶液中As(III)的初始质量浓度为74 μg/L,占总砷质量浓度的11.2%,在加入微生物和葡萄糖后,在缺氧和有氧条件下,As(III)的质量浓度分别为278 μg/L和310 μg/L,占总砷质量浓度的42%和47%.微宇宙实验说明地下水中的土著微生物能将As(V)还原成As(III).
- 砷 /
- 微生物 /
- 地球化学 /
- 氧化还原 /
- 地下水 /
- 河套平原
Abstract: In order to understand the effect of microbial activities on the occurrence of high arsenic groundwater,microcosm experiments were conducted using the indigenous microbe (YH002) isolated from high arsenic groundwater collected from Hetao Plain. The experiments show that the microcosm supplemented with bacteria and glucose,bacterial metabolism can excrete the organic acid which can cause pH decline. Under anoxic conditions,the maximal OD values reached 0.189 on the 7th day and the pH reduced to 6.22. However,under oxic conditions,the maximum of OD value also occurred on the 7th day with the value of 0.286,while pH values reduced to 6.04 at the end of the experiment. The initial concentration of As (III) was 74 μg/L,accounting for 11.2% of the total arsenic,when at the presence of the microbe and glucose,As (III) concentrations were 278 μg/L and 310 μg/L,accounting for 42% and 47% of the total arsenic,in the N₂ and O₂ atmosphere,respectively. The experimental results show that the indigenous microbe is capable of reducing As (V) to As (III) in groundwater.
- arsenic /
- microbes /
- geochemistry /
- redox /
- groundwater /
- Hetao plain

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图 1 采样点位置

Fig. 1. Site of sampling

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图 2 YH002与16S rRNA构建的进化树

Fig. 2. Phylogenetic relationships between 16S rRNA sequence of the YH002 strain and related sequences

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图 3 菌液OD值(600 nm)随土著微生物培养时间的变化

■表示不加微生物不加葡萄糖；●表示加葡萄糖；▲表示加微生物；▼表示加葡萄糖加微生物

Fig. 3. The change of OD value (600 nm) in solution with the time of indigenous microbial culture

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图 4 pH值随土著微生物培养时间的变化

■表示不加微生物不加葡萄糖；●表示加葡萄糖；▲表示加微生物；▼表示加葡萄糖加微生物

Fig. 4. The change of pH value in solution with the time of indigenous microbial culture

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图 5 As(III)含量随土著微生物培养时间的变化

■表示不加微生物不加葡萄糖；●表示加葡萄糖；▲表示加微生物；▼表示加葡萄糖加微生物

Fig. 5. The change of As (III) in solution with the time of indigenous microbial culture

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参考文献(14)

Croal, L.R., Gralnick, J.A., Malasarn, D., et al., 2004. The genetics of geochemistry. Annual Review of Genetics, 38: 175-202. doi: 10.1146/annurev.genet.38.072902.091138
Cullen, W.R., Reimer, K.J., 1989. Arsenic speciation in the environment. Chemical Reviews, 89(4): 713-764. doi: 10.1021/cr00094a002
Duan, M.Y., Xie, Z.M., Wang, Y.X., et al., 2009. Microcosm studies on iron and arsenic mobilization from aquifer sediments under different conditions of microbial activity and carbon source. Environmental Geology, 57: 997-1003. doi: 10.1007/s00254-008-1384-z
Gihring, T.M., Druschel, G.K., Mccleskey, R.B., et al., 2001. Rapid arsenite oxidation by thermus aquaticus and thermus thermophilus: field and laboratory investigations. Environmental Science and Technology, 35: 3857-3862. doi: 10.1021/es010816f
Herbel, M., Fendorf, S., 2006. Biogeochemical processes controlling the speciation and transport of arsenic within iron coated sands. Chemical Geology, 228(1-3): 16-32. doi: 10.1016/j.chemgeo.2005.11.016
Humayoun, S.B., Bano, N., Hollibaugh, J.T., 2003. Depth distribution of microbial diversity in Mono Lake, a meromictic soda lake in California. Applied and Environmental Microbiology, 69: 1030-1042. doi: 10.1128/AEM.69.2.1030-1042.2003
Islam, F.S., Gault, A.G., Boothman, C., et al., 2004. Role of metal-reducing bacteria in arsenic release from Bengal deltasediments. Nature, 430: 68-71. doi: 10.1038/nature02638
Kasan, H.C., 1993. The role of waste activated sludge and bacteria in metal-ion removal from solution. Environmental Science and Technology, 23(1): 79-117.
Katsoyiannis, I., Zouboulis, A., Althoff, H., et al., 2002. As (III) removal from groundwaters using fixed-bed upflow bioreactors. Chemosphere, 47: 325-332. doi: 10.1016/S0045-6535(01)00306-X
Le, X.C., Yalcin, S., Ma, M., 2000. Speciation of submicrogram per liter levels of arsenic in water: on-site species separation integrated with sample collection. Environmental Science and Technology, 34: 2342-1347. doi: 10.1021/es991203u
Menna, P., Hungria, M., Barcellos, F.G., et al., 2006. Molecular phylogeny based on the 16S rRNA gene of elite rhizobial strains used in Brazilian commercial inoculants. Systematic and Applied Microbiology, 29(4): 315-332. doi: 10.1016/j.syapm.2005.12.002
Ng, J.C., Wang, J.P., Shraim, A., 2003. A global health problem caused by arsenic from natural sources. Chemosphere, 52: 1353-1359. doi: 10.1016/S0045-6535(03)00470-3
Oremland, R.S., Stolz, J.F., 2005. Arsenic, microbes and contaminated aquifers. Trends in Microbiology, 13(2): 45-49. doi: 10.1016/j.tim.2004.12.002
Stackebrandt, E., Goebel, B.M., 1994. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. International Journal Systematic Bacteriology, 44(4): 846-849. http://mbe.oxfordjournals.org/cgi/ijlink?linkType=ABST&journalCode=ijs&resid=44/4/846