神农架大九湖不同生境表土磷脂脂肪酸揭示的微生物群落结构差异

赵美玲; 张一鸣; 张志麒; 黄咸雨

doi:10.3799/dqkx.2019.272

神农架大九湖不同生境表土磷脂脂肪酸揭示的微生物群落结构差异

doi: 10.3799/dqkx.2019.272

赵美玲^1, ,,
张一鸣²,
张志麒^{2, 3},
黄咸雨^{1, 2, , ,}

1.
中国地质大学流域关键带演化湖北省重点实验室, 地理与信息工程学院, 湖北武汉 430078
2.
中国地质大学生物地质与环境地质国家重点实验室, 湖北武汉 430078
3.
神农架国家公园管理局, 湖北神农架 442400

基金项目:

国家自然科学基金项目 41877317

中央高校基本科研业务费专项资金项目 CUGCJ1703

中央高校基本科研业务费专项资金项目 CUGQY1902

生物地质与环境地质国家重点实验室自主课题 GBL11612

详细信息

作者简介:
赵美玲(1994-), 硕士研究生, 主要从事土壤微生物脂类研究

通讯作者:
黄咸雨

中图分类号: Q93
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- 被引次数: 0
出版历程
- 收稿日期: 2019-09-10
- 刊出日期: 2020-06-15

Comparison of Microbial Community in Topsoil among Different Habitats in Dajiuhu, Hubei Province: Evidence from Phospholipid Fatty Acids

Zhao Meiling^{1
,
,},
Zhang Yiming²,
Zhang Zhiqi^{2, 3},
Huang Xianyu^{1, 2
, ,
,}

1.
Hubei Key Laboratory of Critical Zone Evolution, School of Geography and Information Engineering, China University of Geosciences, Wuhan 430078, China
2.
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430078, China
3.
Shennongjia National Park Administration, Shennongjia 442400, China

摘要

摘要: 磷脂脂肪酸(phospholipid fatty acid，PLFAs)是活体微生物细胞膜的重要组成部分，微生物通过改变细胞膜中PLFA组成，快速响应环境变化.目前，表土PLFAs研究主要集中于季节和植被群落变化对微生物群落结构影响，对于不同生境下表土PLFAs揭示的微生物群落结构的差异性尚不明确.基于此，对神农架大九湖7种不同生境表土进行PLFAs研究.结果表明，表土样品PLFAs集中分布于C₁₄到C₁₉；除湿生泥炭沼泽和湿生半退化沼泽生境外，其他生境以n16：0为主峰.不同生境的PLFAs含量差异较大，沼泽生境TPLFAs含量是草甸及阔叶林生境下的3~8倍.PLFAs组成还揭示出生境间主要受到pH和含水率的影响，微生物群落结构存在差异.不同生境下表层土壤PLFAs揭示的微生物丰度和群落结构具有一定的相似性及差异性.运用PLFAs对微生物量及微生物群落结构的划分将有助于更好的了解区域生态系统中微生物群落结构的变化，为研究微生物参与碳循环及古生态研究奠定基础.
- 磷脂脂肪酸 /
- 生境 /
- 微生物量 /
- 微生物群落结构 /
- 表土 /
- 地球生物学
Abstract: As an important component of microbial cell membrane,phospholipid fatty acid (PLFAs) can respond sensitively to environmental changes,PLFAs can be altered by microorganisms changing their cell membrane composition by changing their metabolic or nutrient pathways. The current researches on soil PLFAs mainly focus on how changes in seasons and vegetation community affect microbial community structure. It is still not clear how habitats mediate the structure of soil microbial community revealed by topsoil PLFAs. In this study,soil PLFAs compositions were investigated among different habitats (including Sphagnum peat,herb peat,degraded peat,hygrophyte-mesophyte meadow,mesophyte-xeric meadow,xeric meadow,and deciduous broad-leaved forest) in Dajiuhu,Shennongjia. The results show that totally 26 PLFAs with carbon numbers ranging from C₁₄ to C₁₉ are common in the topsoil of the seven habitats. The concentration of total PLFAs in peats is 3-8 times higher than that in meadows. Because of pH and SWC (soil water content) PLFAs also reveal that microbial community structures are different among habitats. The microbial abundance and microbial community structure are similar and different in topsoil under different habitats. The results in this study shed light to better understand the changes of microbial community structure in regional ecosystem,and to facilitate the study of microbe's role in carbon cycle,paleoecology.
- phospholipid fatty acid /
- habitat /
- microbial biomass /
- microbial community structure /
- topsoil /
- geobiology

HTML全文

图 1 大九湖代表性生境表土PLFAs含量分布

a.湿生泥炭沼泽；b.湿生草本沼泽; c.湿生-中生草甸；d.落叶阔叶林

Fig. 1. PLFAs concentration in topsoils collected from typical habitats in Dajiuhu basin

下载: 全尺寸图片幻灯片

图 2 总有机碳含量与总PLFAs含量关系

Fig. 2. The relationship between total organic carbon content and total PLFAs concentration

下载: 全尺寸图片幻灯片

图 3 基于PLFAs的微生物群落结构分析

A.湿生泥炭沼泽；B.湿生草本沼泽；C.湿生半退化沼泽；D.湿生-中生草甸；E.中生-旱生草甸；F.旱生草甸；G.落叶阔叶林

Fig. 3. Analysis of microbial community structure based on PLFAs

下载: 全尺寸图片幻灯片

图 4 RDA(冗余分析)基于PLFAs的微生物群落结构与环境因子关系

Fig. 4. Redundancy analysis between microbial community structure and environmental factors based on PLFAs

下载: 全尺寸图片幻灯片

图 5 来源于革兰氏阴性菌的PLFAs含量与土壤含水率关系

Fig. 5. The relationship between the concentration of gram-negative bacteria derived PLFAs and soil moisture content

下载: 全尺寸图片幻灯片

表 1 不同生境下表土的物理化学性质

Table 1. Physical and chemical properties of topsoil collected from different habitats

生境类型	SWC	pH	NH₃-N	NO₃^--N	C/N	TOC	T
生境类型	(%)	pH	(mg/L)	(mg/L)	C/N	(%)	(℃)
湿生泥炭沼泽(n=4)	94.0	4.1±0.1	2.9±0.7	0.2±0.1	15.5±2.6	19.8±2.0	17.5±3.7
湿生草本沼泽(n =5)	84.0±0.1	4.1±0.3	2.6±0.9	0.1±0.1	18.6±2.0	19.7±3.0	16.6±2.9
湿生半退化沼泽(n =3)	76.0±0.1	3.9±0.2	3.4±1.3	0.2±0.1	16.8±0.6	18.8±5.4	17.7±0.5
湿生-中生草甸(n =3)	51.0±0.1	4.6±0.1	2.5±0.9	0.2±0.2	18.8±5.0	5.7±2.7	18.7±1.7
中生-旱生草甸(n =2)	34.0±0.1	4.7±0.1	3.0±0.3	1.0±0.6	15.4±4.4	6.9±0.8	19.5±0.6
旱生草甸(n =2)	36.0	4.6±0.1	3.4±0.2	1.0±0.4	16.6±6.2	5.8±1.1	20.0
落叶阔叶林(n =3)	48.0	4.8±1.0	3.5±1.6	0.1±0.1	25.2±6.9	9.1±0.7	18.0

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