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    二价铁氧化对铁锰循环功能微生物活性的影响及机制

    屈婧祎 童曼 袁松虎

    屈婧祎, 童曼, 袁松虎, 2021. 二价铁氧化对铁锰循环功能微生物活性的影响及机制. 地球科学, 46(2): 632-641. doi: 10.3799/dqkx.2020.029
    引用本文: 屈婧祎, 童曼, 袁松虎, 2021. 二价铁氧化对铁锰循环功能微生物活性的影响及机制. 地球科学, 46(2): 632-641. doi: 10.3799/dqkx.2020.029
    Qu Jingyi, Tong Man, Yuan Songhu, 2021. Effect and Mechanism of Fe(II) Oxygenation on Activities of Iron and Manganese Cycling Functional Microbes. Earth Science, 46(2): 632-641. doi: 10.3799/dqkx.2020.029
    Citation: Qu Jingyi, Tong Man, Yuan Songhu, 2021. Effect and Mechanism of Fe(II) Oxygenation on Activities of Iron and Manganese Cycling Functional Microbes. Earth Science, 46(2): 632-641. doi: 10.3799/dqkx.2020.029

    二价铁氧化对铁锰循环功能微生物活性的影响及机制

    doi: 10.3799/dqkx.2020.029
    基金项目: 

    国家自然科学基金青年基金项目 41703113

    详细信息
      作者简介:

      屈婧祎(1993-), 女, 硕士研究生, 主要从事铁、锰氧化还原循环的研究.ORCID: 0000-0001-8195-9915.E-mail: 1352442194@qq.com

      通讯作者:

      童曼, ORCID: 0000-0002-0286-0608.E-mail: tongman@cug.edu.cn

    • 中图分类号: P641.3

    Effect and Mechanism of Fe(II) Oxygenation on Activities of Iron and Manganese Cycling Functional Microbes

    • 摘要: 地质微生物是沉积环境中铁、锰氧化还原循环的主要驱动因子,铁锰共存环境中二价铁氧化对不同铁、锰循环功能微生物活性的影响差异和机制尚不清楚.以铁还原菌Shewanella oneidensis MR-1、铁氧化菌Pseudogulbenkiania sp.strain 2002、锰氧化菌Pseudomonas putida MnB1和Leptothrix discophora SS-1作为代表性的铁、锰循环功能微生物,利用平板计数、荧光显微镜等手段探究了Fe(II)氧化对功能微生物活性的影响差异及机制.结果表明0.05 mM Fe2+氧化60 min可使MR-1和MnB1的活菌数量降低4~5个数量级,SS-1及S.2002无显著失活.Fe(II)氧化产生的吸附态。OH和胞内。OH是细菌失活的主要原因,胞外H2O2、胞外游离态。OH和三价铁氧化物是细菌失活的次要原因,SS-1及S.2002产生了氧化应激反应,成功抵御了活性氧化物种.

       

    • 图  1  Fe(II)氧化对Pseudogulbenkiania sp. strain 2002、Shewanella oneidensis MR-1、Pseudomonas putida MnB1和Leptothrix discophora SS1活性的影响

      Fig.  1.  Effects of Fe(II) oxygenation on the activities of Pseudogulbenkiania sp. strain 2002, Shewanella oneidensis MR-1, Pseudomonas putida MnB1 and Leptothrix discophora SS1

      图  2  不同氧分压下Fe(II)氧化对Pseudomonas putida MnB1细菌活性的影响

      Fig.  2.  Effect of Fe(II) oxygenation on the activities of Pseudomonas putida MnB1 under various oxygen partial pressure

      图  3  Fe(II)氧化影响前后细菌的荧光显微镜照片

      Fig.  3.  Florescence microscope photos of bacteria before and after Fe(II) oxygenation

      图  4  Fe2+的氧化动力学(a、b)和H2O2、•OH累计浓度(c、d)

      Fig.  4.  The oxidation kinetics of Fe2+ (a, b) and the accumulative concentrations of H2O2 and •OH (c, d)

      图  5  H2O2对细菌活性的影响

      Fig.  5.  Effect of H2O2 on the activities of bacteria

      图  6  H2O2影响前后细菌的荧光显微镜照片

      Fig.  6.  Florescence microscope photos of bacteria before and after the reaction with H2O2

      图  7  体系中H2O2浓度变化

      Fig.  7.  Variations of H2O2 concentration in bacteria suspensions

      图  8  加入活性氧淬灭剂对细菌活性变化的影响

      Fig.  8.  Effect of •OH quencher on the variations of bacteria activities

      图  9  三价铁氧化物对MR-1(a)和MnB1(b)活性的影响

      Fig.  9.  Effect of Fe(III) oxides on the activities of MR-1 (a) and MnB1 (b)

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    • 收稿日期:  2019-11-14
    • 刊出日期:  2021-02-15

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