Influence of Bioreduction of Arsenic-Bearing Goethite by Bacteria under Sulfur Mediation on Migration and Transformation of Arsenic
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摘要: 硫在铁和砷的生物地球化学循环中发挥着重要作用，但地下水系统中硫循环的中间产物S(0)对细菌转化铁和砷的影响尚不清楚.采用室内模拟实验，研究硫参与下细菌D2201对液相和载砷针铁矿中Fe(III)和As(V)的还原作用.结果表明：细菌D2201具有很强的铁还原能力，可以将液相中74%的Fe(III)还原；加入硫后，细菌还原S(0)产生的S(-II)使铁还原率提高到94%.但是，硫没有明显影响细菌对砷的还原.在实验初期，细菌明显加速了载砷针铁矿的还原，最终还原释放到液相中的Fe(II)浓度为32.12 μmol/L；硫的加入增强了细菌对载砷针铁矿的还原，还原溶解的Fe(II)增加至284.13 μmol/L，同时，砷的释放量也增加了1.6倍.这些结果表明硫显著促进了细菌对针铁矿的还原溶解并加速砷的释放.XRD和SEM-EDS结果显示，细菌还原针铁矿但不改变其矿相，而硫的加入也仅使矿物发生一定程度的团聚，并没有使其转变为其他矿物，也未导致砷的再吸附.Abstract: Sulfur plays an important role in the biogeochemical cycle of iron and arsenic. But the effect of S(0),an intermediate product of the sulfur cycle in groundwater systems,on biotransformation of iron and arsenic remains unexplored. Laboratory simulation experiments were conducted to investigate the reduction of Fe(III) and As(V) in the liquid phase and arsenic-bearing goethite by bacteria D2201 with sulfur. The results show that 74% of Fe(III) in the liquid phase were reduced by the strain D2201 with strong iron-reducing potential. When sulfur was added,S(-II) from the bacteria reducing S(0) increased the iron reduction rate to 94%. However,the bacterial reduction of arsenic was not accelerated significantly by added sulfur. Fe(III) in arsenic-bearing goethite was reduced rapidly by the strain D2201 at the beginning of the experiment. In the experiment,32.12 μmol/L Fe(II) was released in the liquid phase. The added sulfur enhanced the bacterial reduction of arsenic-bearing goethite,and the released Fe(II) increased to 284.13 μmol/L. At the same time,the concentration of arsenic released into solution increased 1.6 times. The results indicate that sulfur significantly promoted the bacterial reduction and dissolution of arsenic-bearing goethite and accelerated the release of arsenic. It was shown by XRD and SEM-EDS that the mineral phase of iron mineral reduced by the strain D2201 was not changed. Under the condition of sulfur,the mineral only has a certain degree of agglomeration,not transform to other minerals. And arsenic was not adsorbed by the mineral once again.
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Key words:
- iron reducing bacteria /
- goethite /
- sulfur /
- arsenic /
- migration and transformation /
- geochemistry
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图 1 细菌还原铁过程中硫对Fe(II)含量的影响
Fig. 1. Changes of Fe(II) content during the effect of sulfur on the reduction of iron by bacteria
图 2 细菌还原砷过程中硫对砷含量和形态变化的影响
Fig. 2. Changes of arsenic content and species during the effect of sulfur on the reduction of arsenic by bacteria
图 3 硫作用下细菌对载砷针铁矿还原过程中液相Fe(II)、S(-II)、As含量变化
Fig. 3. Changes of Fe(II), S(-II) and As contents in liquid phase during the effect of sulfur on the reduction of arsenic-bearing goethite by bacteria
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