湖北清江和尚洞滴水微生物群落随空间和季节的变化

赵锐; 王红梅; 刘倩瑛; 曾翠平

doi:10.3799/dqkx.2010.105

Volume 35 Issue 6

Jun. 2010

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Article Navigation > Earth Science > 2010 > 35(6): 899-907

ZHAO Rui, WANG Hong-mei, LIU Qian-ying, ZENG Cui-ping, 2010. Spatial and Seasonal Variations of Microbial Communities in Dripping Water from the Heshang Cave, Central China. Earth Science, 35(6): 899-907. doi: 10.3799/dqkx.2010.105

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ZHAO Rui, WANG Hong-mei, LIU Qian-ying, ZENG Cui-ping, 2010. Spatial and Seasonal Variations of Microbial Communities in Dripping Water from the Heshang Cave, Central China. Earth Science, 35(6): 899-907. doi: 10.3799/dqkx.2010.105

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Spatial and Seasonal Variations of Microbial Communities in Dripping Water from the Heshang Cave, Central China

doi: 10.3799/dqkx.2010.105

1.
Key Laboratory of Biogeology and Environmental Geology of Ministry of Education, China University of Geosciences, Wuhan 430074, China
2.
School of Earth and Space Sciences, Peking University, Beijing 100871, China

Received Date: 2009-12-15
Publish Date: 2010-11-01

Abstract

Abstract

Dripping water was seasonally collected from several sites (denoted as site 1D, 3D, 4D and 5D toward the cave entrance respectively) inside the oligotrophic Heshang cave, Central China, to detect the metabolic activities of microbial communities in response to rainfall and temperature. Viable counting method, the DAPI staining technique as well as Biolog Eco microplates were exploited for microbial counting and metabolic changes of microbial communities. Results confirm the existence of microbes in the dripping water though the microbial biomass is as low as that in the deep sea water column. The metabolic activities of microbial communities varied with sites as indicated by the average well color development (AWCD) values in Biolog ECO microplates. Microbes in site 1D showed the strongest metabolic activities followed by those in site 3D. Microbial communities in the sites near the cave entrance, such as site 4D and site 5D, however, displayed the weakest metabolic activities as shown by the invariant AWCD values after 24-hour incubation. Similar microbial diversity indices were observed between different sites such as site 1D and site 3D, and even among different seasons. This indicates the presence of a fairly stable microbial diversity in the dripping water, probably due to the oligotrophic condition in the cave. However, the carbon utilization pattern of microbial communities in site 1D differed greatly among seasons; the pattern in Sept. 2008 is distinguishable from those in other sampling periods. The seasonal variation in carbon utilization might arises from the seasonal changes of dissolved organic matter or the changes in the flow rate of dripping water. Noticeably, such kind of seasonal variation in the carbon utilization was not reproduced in site 3D. Yet we need further investigations to determine the relationship between the changes in microbial community and the climate factors such as temperature and rainfall.
- karst cave,
- climate change,
- microbiology,
- community diversity

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Spatial and Seasonal Variations of Microbial Communities in Dripping Water from the Heshang Cave, Central China

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