固氮鱼腥藻介导As(Ⅲ)氧化及其对铵氮输入的响应

钟兆淇; 谢作明; 毛青; 赵欣鑫; 刘太坤

doi:10.3799/dqkx.2022.079

Volume 49 Issue 5

May 2024

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Article Navigation > Earth Science > 2024 > 49(5): 1920-1930

Zhong Zhaoqi, Xie Zuoming, Mao Qing, Zhao Xinxin, Liu Taikun, 2024. Nitrogen-Fixing Anabaena sp. Mediated As(Ⅲ) Oxidation and Its Response to Ammonium Input. Earth Science, 49(5): 1920-1930. doi: 10.3799/dqkx.2022.079

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Zhong Zhaoqi, Xie Zuoming, Mao Qing, Zhao Xinxin, Liu Taikun, 2024. Nitrogen-Fixing Anabaena sp. Mediated As(Ⅲ) Oxidation and Its Response to Ammonium Input. Earth Science, 49(5): 1920-1930. doi: 10.3799/dqkx.2022.079

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Nitrogen-Fixing Anabaena sp. Mediated As(Ⅲ) Oxidation and Its Response to Ammonium Input

doi: 10.3799/dqkx.2022.079

Zhong Zhaoqi^{1
,
,},
Xie Zuoming^{1, 2
,
,},
Mao Qing¹,
Zhao Xinxin¹,
Liu Taikun¹

1.
School of Environmental Studies, China University of Geosciences, Wuhan 430078, China
2.
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430078, China

Received Date: 2022-03-02
Available Online: 2024-06-04
Publish Date: 2024-05-25

Abstract

Abstract

Ammonium plays an important role in the biogeochemical cycle of arsenic, but the mechanism of ammonium input on algae-mediated As(Ⅲ) bio-oxidation remains unexplored. The arsenic-resistant cyanobacteria Anabaena sp. was used in this study. Through laboratory simulation experiments, the toxicity, oxidation of different As(Ⅲ)concentrations mediated by Anabaena sp. was investigated. The influences of the input of exogenous ammonium on the growth of Anabaena sp. and the oxidation of As(Ⅲ) were also explored. The results show that the IC₅₀ value of Anabaena sp. was 15.59 mg/L, and 0.1 mg/L, 1 mg/L and 10 mg/L As(Ⅲ) were completely oxidized to As(Ⅴ) within 1 d, 2 d and 7 d, respectively, after inoculating Anabaena sp., With the concentration of As(Ⅲ) increased, the nitrogen fixation effect of Anabaena sp. is enhanced. Furthermore, the growth of Anabaena sp. was promoted within 234 mg/L NH₄⁺. With the increase of NH₄⁺ concentration from 0 mg/L to 1 mg/L, 45 mg/L and 234 mg/L, 1 mg/L As(Ⅲ) was completely oxidized at 48 h, 36 h, 24 h and 12 h, respectively, as the concentration of NH₄⁺ increases, the adsorption of Anabaena sp. to arsenic increases, and the oxidation of As(Ⅲ) accelerates. The research results help to interpret the role of ammonium nitrogen in the process of arsenic biotransformation in natural waters.
- anabaena sp,
- arsenite,
- ammonium,
- toxicity,
- oxidation,
- adsorption,
- geochemistry

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References(42)

References

Awoyemi, O. M., Subbiah, S., Velazquez, A., et al., 2020. Nitrate-N-Mediated Toxicological Responses of Scenedesmus Acutus and Daphnia Pulex to Cadmium, Arsenic and Their Binary Mixture (Cd/As_mix) at Environmentally Relevant Concentrations. Journal of Hazardous Materials, 400: 123189. https://doi.org/10.1016/j.jhazmat.2020.123189

Bahar, M. M., Megharaj, M., Naidu, R., 2013. Toxicity, Transformation and Accumulation of Inorganic Arsenic Species in a Microalga Scenedesmus sp. Isolated from Soil. Journal of Applied Phycology, 25(3): 913-917. https://doi.org/10.1007/s10811-012-9923-0

Berman-Frank, I., Lundgren, P., Falkowski, P., 2003. Nitrogen Fixation and Photosynthetic Oxygen Evolution in Cyanobacteria. Research in Microbiology, 154(3): 157-164. https://doi.org/10.1016/S0923-2508(03)00029-9

Böhme, H., 1998. Regulation of Nitrogen Fixation in Heterocyst-Forming Cyanobacteria. Trends in Plant Science, 3(9): 346-351. https://doi.org/10.1016/s1360-1385(98)01290-4

Chakraborty, A., Aziz Chowdhury, A., Bhakat, K., et al., 2019. Elevated Level of Arsenic Negatively Influences nifH Gene Expression of Isolated Soil Bacteria in Culture Condition as Well as Soil System. Environmental Geochemistry and Health, 41(5): 1953-1966. https://doi.org/10.1007/s10653-019-00261-2

Che, F. F., Du, M. M., Yan, C. Z., 2018. Arsenate Biotransformation by Microcystis Aeruginosa under Different Nitrogen and Phosphorus Levels. Journal of Environmental Sciences (China), 66: 41-49. https://doi.org/10.1016/j.jes.2017.05.041

Collos, Y., Harrison, P. J., 2014. Acclimation and Toxicity of High Ammonium Concentrations to Unicellular Algae. Marine Pollution Bulletin, 80(1/2): 8-23. https://doi.org/10.1016/j.marpolbul.2014.01.006

Dawodu, M. O., Akpomie, K. G., 2016. Evaluating the Potential of a Nigerian Soil as an Adsorbent for Tartrazine Dye: Isotherm, Kinetic and Thermodynamic Studies. Alexandria Engineering Journal, 55(4): 3211-3218. https://doi.org/10.1016/j.aej.2016.08.008

Fang, J. H., Xie, Z. M., Wang, J., et al., 2021. Bacterially Mediated Release and Mobilization of As/Fe Coupled to Nitrate Reduction in a Sediment Environment. Ecotoxicology and Environmental Safety, 208: 111478. https://doi.org/10.1016/j.ecoenv.2020.111478

Hussain, M. M., Wang, J. X., Bibi, I., et al., 2021. Arsenic Speciation and Biotransformation Pathways in the Aquatic Ecosystem: The Significance of Algae. Journal of Hazardous Materials, 403: 124027. https://doi.org/10.1016/j.jhazmat.2020.124027

Jana, A., Bhattacharya, P., Swarnakar, S., et al., 2015. Anabaena sp. Mediated Bio-Oxidation of Arsenite to Arsenate in Synthetic Arsenic (Ⅲ) Solution: Process Optimization by Response Surface Methodology. Chemosphere, 138: 682-690. https://doi.org/10.1016/j.chemosphere.2015.07.055

Jiamali, J., Maimaiti, G., Tumier, A., 2019. Study on Characteristics of Tolerance and Absorption of Four Heavy Metals by Three Photobionts. Acta Botanica Boreali-Occidentalia Sinica, 39(7): 1230-1240 (in Chinese with English abstract).

Kumar, A., Bera, S., 2020. Revisiting Nitrogen Utilization in Algae: A Review on the Process of Regulation and Assimilation. Bioresource Technology Reports, 12: 100584. https://doi.org/10.1016/j.biteb.2020.100584

Levy, J. L., Stauber, J. L., Adams, M. S., et al., 2005. Toxicity, Biotransformation, and Mode of Action of Arsenic in Two Freshwater Microalgae (Chlorella sp. and Monoraphidium Arcuatum). Environmental Toxicology and Chemistry, 24(10): 2630-2639. https://doi.org/10.1897/04-580r.1

Li, X. T., Li, W., Zhai, J., et al., 2019. Effect of Ammonium Nitrogen on Microalgal Growth, Biochemical Composition and Photosynthetic Performance in Mixotrophic Cultivation. Bioresource Technology, 273: 368-376. https://doi.org/10.1016/j.biortech.2018.11.042

Lichtenthaler, H. K., 1987. Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes. Methods in Enzymology. Elsevier, Amsterdam, 350-382. https://doi.org/10.1016/0076-6879(87)48036-1

Liu, J. Q., Sun, Z. Q., Lavoie, M., et al., 2015. Ammonium Reduces Chromium Toxicity in the Freshwater Alga Chlorella Vulgaris. Applied Microbiology and Biotechnology, 99(7): 3249-3258. https://doi.org/10.1007/s00253-014-6218-1

Ministry of Environmental Protection of the People's Republic of China, 2010. Water Quality Determination of Ammonia Nitrogen Determination by Nessler's Reagent Dectrophotometry (HJ 535-2009). China Environmental Science Press, Beijing (in Chinese).

Miyashita, S. I., Murota, C., Kondo, K., et al., 2016. Arsenic Metabolism in Cyanobacteria. Environmental Chemistry, 13(4): 577. https://doi.org/10.1071/en15071

Ospina-Betancourth, C., Acharya, K., Sanabria, J., et al., 2021. Low Inhibitory Effect of Ammonia on the Nitrogen-Fixing Activity of a Sludge Enriched with Nitrogen-Fixing Bacteria. Bioresource Technology Reports, 14: 100655. https://doi.org/10.1016/j.biteb.2021.100655

Patel, A., Tiwari, S., Prasad, S. M., 2018. Toxicity Assessment of Arsenate and Arsenite on Growth, Chlorophyll a Fluorescence and Antioxidant Machinery in Nostoc Muscorum. Ecotoxicology and Environmental Safety, 157: 369-379. https://doi.org/10.1016/j.ecoenv.2018.03.056

Patel, A., Tiwari, S., Prasad, S. M., 2021. Effect of Time Interval on Arsenic Toxicity to Paddy Field Cyanobacteria as Evident by Nitrogen Metabolism, Biochemical Constituent, and Exopolysaccharide Content. Biological Trace Element Research, 199(5): 2031-2046. https://doi.org/10.1007/s12011-020-02289-3

Pokhrel, D., Viraraghavan, T., 2008. Arsenic Removal from an Aqueous Solution by Modified A. Niger Biomass: Batch Kinetic and Isotherm Studies. Journal of Hazardous Materials, 150(3): 818-825. https://doi.org/10.1016/j.jhazmat.2007.05.041

Qu, G. Y., Li, M. J., Zheng, J. H., et al., 2022. The Promoting Effect and Mechanism of Nitrogen Conversion in the Sediments of Polluted Lake on the Degradation of Organic Pollutants. Earth Science, 47(2): 652-661 (in Chinese with English abstract).

Ruan, Y. H., Fang, X., Guo, T. Y., et al., 2022. Metabolic Reprogramming in the Arsenic Carcinogenesis. Ecotoxicology and Environmental Safety, 229: 113098. https://doi.org/10.1016/j.ecoenv.2021.113098

Tabaraki, R., Heidarizadi, E., 2018. Simultaneous Biosorption of Arsenic (Ⅲ) and Arsenic (Ⅴ): Application of Multiple Response Optimizations. Ecotoxicology and Environmental Safety, 166: 35-41. https://doi.org/10.1016/j.ecoenv.2018.09.063

Wang, J., Xie, Z. M., Wang, J., et al., 2021. Influence of Bioreduction of Arsenic-Bearing Goethite by Bacteria under Sulfur Mediation on Migration and Transformation of Arsenic. Journal of Earth Science, 46(2): 642-651 (in Chinese with English abstract).

Wang, N. X., Huang, B., Xu, S., et al., 2014. Effects of Nitrogen and Phosphorus on Arsenite Accumulation, Oxidation, and Toxicity in Chlamydomonas Reinhardtii. Aquatic Toxicology, 157: 167-174. https://doi.org/10.1016/j.aquatox.2014.10.012

Wang, N. X., Li, Y., Deng, X. H., et al., 2013a. Toxicity and Bioaccumulation Kinetics of Arsenate in Two Freshwater Green Algae under Different Phosphate Regimes. Water Research, 47(7): 2497-2506. https://doi.org/10.1016/j.watres.2013.02.034

Wang, Z. H., Luo, Z. X., Yan, C. Z., 2013b. Accumulation, Transformation, and Release of Inorganic Arsenic by the Freshwater Cyanobacterium Microcystis Aeruginosa. Environmental Science and Pollution Research, 20(10): 7286-7295. https://doi.org/10.1007/s11356-013-1741-7

Wang, S. Z., Zhang, D. Y., Pan, X. L., 2012. Effects of Arsenic on Growth and Photosystem Ⅱ (PSII) Activity of Microcystis Aeruginosa. Ecotoxicology and Environmental Safety, 84: 104-111. https://doi.org/10.1016/j.ecoenv.2012.06.028

Wang, Y., Wang, S., Xu, P. P., et al., 2015. Review of Arsenic Speciation, Toxicity and Metabolism in Microalgae. Reviews in Environmental Science and Bio/Technology, 14(3): 427-451. https://doi.org/10.1007/s11157-015-9371-9

Wang, Z. H., Fu, Y., Wang, L. L., 2021. Abiotic Oxidation of Arsenite in Natural and Engineered Systems: Mechanisms and Related Controversies over the Last Two Decades (1999-2020). Journal of Hazardous Materials, 414: 125488. https://doi.org/10.1016/j.jhazmat.2021.125488

Xue, X. M., Yan, Y., Xiong, C., et al., 2017. Arsenic Biotransformation by a Cyanobacterium Nostoc sp. PCC 7120. Environmental Pollution, 228: 111-117. https://doi.org/10.1016/j.envpol.2017.05.005

Zhang, S. Y., Rensing, C., Zhu, Y. G., 2014. Cyanobacteria-Mediated Arsenic Redox Dynamics is Regulated by Phosphate in Aquatic Environments. Environmental Science & Technology, 48(2): 994-1000. https://doi.org/10.1021/es403836g

Zhao, X. C., Tan, X. B., Yang, L. B., et al., 2019. Cultivation of Chlorella Pyrenoidosa in Anaerobic Wastewater: The Coupled Effects of Ammonium, Temperature and pH Conditions on Lipids Compositions. Bioresource Technology, 284: 90-97. https://doi.org/10.1016/j.biortech.2019.03.117

Zhao, X. X., Li, Y. L., Li, Y. W., et al., 2020. Effects of Increased Nitrogen Deposition and Anthropogenic Perturbation on Soil Respiration in a Semiarid Grassland. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 36(15): 120-127 (in Chinese with English abstract).

吉米拉木·加马力, 古海尼沙·买买提, 艾尼瓦尔·吐米尔, 2019.3种地衣体内藻类对4种重金属离子的耐受能力及其吸附特性研究. 西北植物学报, 39(7): 1230-1240. https://www.cnki.com.cn/Article/CJFDTOTAL-DNYX201907012.htm

中华人民共和国环境保护部, 2010. 水质氨氮的测定纳氏试剂分光光度法: HJ 535—2009. 北京: 中国环境科学出版社.

屈国颖, 李民敬, 郑剑涵, 等, 2022. 受污染湖泊沉积物中氮素转化对有机污染物降解的促进效应与机制. 地球科学, 47(2): 652-661. doi: 10.3799/dqkx.2021.095

王晶, 谢作明, 王佳, 等, 2021. 硫介导细菌还原载砷铁矿对砷迁移转化的影响. 地球科学, 46(2): 642-651. doi: 10.3799/dqkx.2020.054

赵欣鑫, 李玉霖, 李有文, 等, 2020. 氮沉降增加和人类干扰对半干旱草地土壤呼吸的影响. 农业工程学报, 36(15): 120-127. https://www.cnki.com.cn/Article/CJFDTOTAL-NYGU202015016.htm

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