湖南冷水江锡矿山尾矿库细菌富集群落与辉锑矿的相互作用及其群落演替

王宇鑫; 邬梦晓俊; 王纬琦; 李旻; 雷静文; 王红梅

doi:10.3799/dqkx.2022.287

Volume 48 Issue 11

Nov. 2023

Turn off MathJax

Article Contents

Article Navigation > Earth Science > 2023 > 48(11): 4311-4320

Wang Yuxin, Wu Mengxiaojun, Wang Weiqi, Li Min, Lei Jingwen, Wang Hongmei, 2023. Interaction between Stibnite and Microbial Communities Enriched from Tailings at Xikuangshan Coupling with Bacterial Community Succession. Earth Science, 48(11): 4311-4320. doi: 10.3799/dqkx.2022.287

Citation:

Wang Yuxin, Wu Mengxiaojun, Wang Weiqi, Li Min, Lei Jingwen, Wang Hongmei, 2023. Interaction between Stibnite and Microbial Communities Enriched from Tailings at Xikuangshan Coupling with Bacterial Community Succession. Earth Science, 48(11): 4311-4320. doi: 10.3799/dqkx.2022.287

Citation:

Wang Yuxin, Wu Mengxiaojun, Wang Weiqi, Li Min, Lei Jingwen, Wang Hongmei, 2023. Interaction between Stibnite and Microbial Communities Enriched from Tailings at Xikuangshan Coupling with Bacterial Community Succession. Earth Science, 48(11): 4311-4320. doi: 10.3799/dqkx.2022.287

PDF( 2534 KB)

Interaction between Stibnite and Microbial Communities Enriched from Tailings at Xikuangshan Coupling with Bacterial Community Succession

doi: 10.3799/dqkx.2022.287

1.
School of Environmental Studies, China University of Geosciences, Wuhan 430078, China
2.
State Key Laboratory of Geobiology and Environmental Geology, China University of Geosciences, Wuhan 430078, China

Received Date: 2022-04-10
Available Online: 2023-11-30
Publish Date: 2023-11-25

Abstract

Abstract

To better understand microbial dissolution and oxidation of stibnite at the community level, microbial communities were enriched from the tailings in the Xikuangshan tailings pond, Hunan Province, which were further used to study microbial interaction with stibnite. pH, total Sb, Sb(Ⅴ), Sb(Ⅲ) and SO₄^2- were measured periodically. 16S rRNA high-throughput sequencing and X-ray powder diffractometer (XRD) were exploited to detect compositions of microbial communities and mineral phases, respectively. The results show that microbial communities can grow with sodium lactate and increase the pH of the solution under aerobic condition, which facilitated the dissolution of stibnite. The concentration of Sb(Ⅴ) accumulated in the solution due to the oxidation of the released Sb(Ⅲ), with an decrease after the 12^th day. The Sb(Ⅲ) concentration in the solution increased after the 9^th day and was a little bit higher than that of Sb(Ⅴ) at the end of the experiments. The incomplete oxidation of Sb(Ⅲ) may result from the low relative abundances of antimony-oxidizing bacteria such as Paracoccus and Bosea in the enriched culture. The variation of solution chemistry, particularly the Sb concentration, exerted strong selection on microbial communities. Microbial communities were dominated by Herbaspirillum sp., with strong resistance to metals, such as and Sb. However their relative abundances decreased after 11 days' interaction potentially due to the accumulation of Sb in the system. Secondary Sb(Ⅴ)-bearing mineral, sodium antimonite, was detected at the end of the experiments in the biotic systems, which matched well with the decrease of the concentration of Sb(Ⅴ) in the solution. Our results further confirm the mechanism of microbial dissolution of stibnite followed by oxidation and transformation of minerals at the community level, which provides new insights of understanding the impact of microbial communities on the dissolution of Sb-bearing secondary minerals, oxidation, migration and formation of antimony in natural environments.
- Xikuangshan antimony deposit,
- tailing dam,
- interaction between microbe and mineral,
- stibnite,
- antimony oxidation,
- microbial community succession,
- biogeology,
- geochemistry,
- environmental geology

FullText(HTML)

References(38)

References

Ali, A., Li, M., Su, J., et al., 2022. Brevundimonas diminuta Isolated from Mines Polluted Soil Immobilized Cadmium (Cd²⁺) and Zinc (Zn²⁺) through Calcium Carbonate Precipitation: Microscopic and Spectroscopic Investigations. Science of Total Environment, 813: 152668. https://doi.org/10.1016/j.scitotenv.2021.152668

Biver, M., Shotyk, W., 2012. Stibnite (Sb₂S₃) Oxidative Dissolution Kinetics from pH 1 to 11. Geochimica et Cosmochimica Acta, 79: 127-139. https://doi.org/10.1016/j.gca.2011.11.033

Caporaso, J. G., Kuczynski, J., Stombaugh, J., et al., 2010. QIIME Allows Analysis of High-Throughput Community Sequencing Data. Gut Microbes, 7(5): 335-336. https://doi.org/10.1038/nmeth.f.303

Edgar, R. C., 2013. UPARSE: Highly Accurate OTU Sequences from Microbial Amplicon Reads. Frontiers in Plant Science, 10(10): 996-998. https://doi.org/10.1038/nmeth.2604

Edgar, R. C., Haas, B. J., Clemente, J. C., et al., 2011. UCHIME Improves Sensitivity and Speed of Chimera Detection. Bioinformatics, 27(16): 2194-2200. https://doi.org/10.1093/bioinformatics/btr381

Fang, Q., Huang, T., Wang, N., et al., 2021. Effects of Herbaspirillum sp. P5-19 Assisted with Alien Soil Improvement on the Phytoremediation of Copper Tailings by Vetiveria zizanioides L. Environmental Science and Pollution Research, 28(45): 64757-64768.

Fu, Z., Wu, F., Mo, C., et al., 2016. Comparison of Arsenic and Antimony Biogeochemical Behavior in Water, Soil and Tailings from Xikuangshan, China. Science of the Total Environment, 539: 97-104. https://doi.org/10.1016/j.scitotenv.2015.08.146

Govarthanan, M., Lee, G. W., Park, J. H., et al., 2014. Bioleaching Characteristics, Influencing Factors of Cu Solubilization and Survival of Herbaspirillum sp. GW103 in Cu Contaminated Mine Soil. Chemosphere, 109: 42-48. https://doi.org/10.1016/j.chemosphere.2014.02.054

Hamamura, N., Fukushima, K., Itai, T, 2013. Identification of Antimony- and Arsenic-Oxidizing Bacteria Associated with Antimony Mine Tailing. Microbes and Environments, 28(2): 257-263. https://doi.org/10.1264/jsme2.me12217

Hamamura, N., Nakajima, N., Yamamura, S., 2020. Draft Genome Sequence of the Antimony-Oxidizing Pseudomonas sp. Strain SbOxS1, Isolated from Stibnite Mine Tailing Soil. Microbiology Resource Announcements, 9(49): e01218-20. https://doi.org/10.1128/MRA.01218-20

He, M., Wang, X., Wu, F., et al., 2012. Antimony Pollution in China. Science of Total Environment, 421/422: 41-50. https://doi.org/10.1016/j.scitotenv.2011.06.009

Jiang, N., Li, X. Q., Zhou, A. G., et al., 2020. Effect of pH Value and Fe(Ⅲ) on the Oxidative Dissolution of Stibnite. Bulletin of Geological Science and Technology, 39(4): 76-84 (in Chinese with English abstract).

Li, J., Wang, Q., Li, M., et al., 2015. Proteomics and Genetics for Identification of a Bacterial Antimonite Oxidase in Agrobacterium tumefaciens. Environmental Science & Technology, 49(10): 5980-5989. https://doi.org/10.1021/es506318b

Li, J., Wang, Q., Zhang, S. Z., et al., 2013. Phylogenetic and Genome Analyses of Antimony-Oxidizing Bacteria Isolated from Antimony Mined Soil. International Biodeterioration & Biodegradation, 76: 76-80. https://doi.org/10.1016/j.ibiod.2012.06.009

Li, Y. B., Lin, H. Z., Gao, P., et al., 2022. Synergistic Impacts of Arsenic and Antimony Co-Contamination on Diazotrophic Communities. Microbial Ecology, 84(1): 44-58. https://doi.org/10.1007/s00248-021-01824-6

Lialikova, N. N., 1974. Stibiobacter senarmontii: A New Microorganism Oxidizing Antimony. Iranian Journal of Public Health, 43(6): 941-948.

Lu, X., Zhang, Y., Liu, C., et al., 2018. Characterization of the Antimonite- and Arsenite-Oxidizing Bacterium Bosea sp. AS-1 and Its Potential Application in Arsenic Removal. Journal of Hazardous Materials, 359: 527-534. https://doi.org/10.1016/j.jhazmat.2018.07.112

Magoč, T., Salzberg, S. L, 2011. FLASH: Fast Length Adjustment of Short Reads to Improve Genome Assemblies. Bioinformatics, 27(21): 2957-2963. https://doi.org/10.1093/bioinformatics/btr507

Multani, R. S., Feldmann, T., Demopoulos, G. P., 2016. Antimony in the Metallurgical Industry: A Review of Its Chemistry and Environmental Stabilization Options. Hydrometallurgy, 164: 141-153. https://doi.org/10.1016/j.hydromet.2016.06.014

Praburaman, L., Park, S. H., Cho, M., et al., 2017. Significance of Diazotrophic Plant Growth-Promoting Herbaspirillum sp. GW103 on Phytoextraction of Pb and Zn by Zea Mays L. Environmental Science and Pollution Research, 24(3): 3172-3180.

Quast, C., Pruesse, E., Yilmaz, P., et al., 2012. The SILVA Ribosomal RNA Gene Database Project: Improved Data Processing and Web-Based Tools. Nucleic Acids Research, 41(D1): D590-D596. https://doi.org/10.1093/nar/gks1219

Rathi, M., Yogalakshmi, K. N., 2021. Brevundimonas diminuta MYS6 Associated Helianthus annuus L. for Enhanced Copper Phytoremediation. Chemosphere, 263: 128195. https://doi.org/10.1016/j.chemosphere.2020.128195

Rhine, E. D., Phelps, C. D., Young, L. Y., 2006. Anaerobic Arsenite Oxidation by Novel Denitrifying Isolates. Environmental Microbiology, 8(5): 899-908. https://doi.org/10.1111/j.1462-2920.2005.00977.x

Sforza, E., Pastore, M., Sanchez, S. S., et al., 2018. Bioaugmentation as a Strategy to Enhance Nutrient Removal: Symbiosis between Chlorella protothecoides and Brevundimonas diminuta. Bioresource Technology Reports, 4: 153-158. https://doi.org/10.1016/j.biteb.2018.10.007

Shi, Z., Cao, Z., Qin, D., et al., 2013. Correlation Models between Environmental Factors and Bacterial Resistance to Antimony and Copper. PLoS One, 8(10): e78533. https://doi.org/10.1371/journal.pone.0078533

Singh, N., Marwa, N., Mishra, S. K., et al., 2016. Brevundimonas Diminuta Mediated Alleviation of Arsenic Toxicity and Plant Growth Promotion in Oryza Sativa L. Ecotoxicology and Environmental Safety, 125: 25-34. https://doi.org/10.1016/j.ecoenv.2015.11.020

Singh, S., Kumar, V., Gupta, P., et al., 2021. The Synergy of Mercury Biosorption through Brevundimonas sp. IITISM22: Kinetics, Isotherm, and Thermodynamic Modeling. Journal of Hazardous Materials, 415: 125653. https://doi.org/10.1016/j.jhazmat.2021.125653

Skeaff, J. M., Beaudoin, R., Wang, R., et al., 2013. Transformation/Dissolution Examination of Antimony and Antimony Compounds with Speciation of the Transformation/Dissolution Solutions. Integrated Environmental Assessment and Management, 9(1): 98-113. https://doi.org/10.1002/ieam.1339

Sun, X. X., Kong, T. L., Häggblom, M. M., et al., 2020. Chemolithoautotropic Diazotrophy Dominates the Nitrogen Fixation Process in Mine Tailings. Environmental Science & Technology, 54(10): 6082-6093. https://doi.org/10.1021/acs.est.9b07835

Sun, X. X., Li, B. Q., Han, F., et al., 2019. Impacts of Arsenic and Antimony Co-Contamination on Sedimentary Microbial Communities in Rivers with Different Pollution Gradients. Microbial Ecology, 78(3): 589-602. https://doi.org/10.1007/s00248-019-01327-5

Terry, L. R., Kulp, T. R., Wiatrowski, H., et al., 2015. Microbiological Oxidation of Antimony (Ⅲ) with Oxygen or Nitrate by Bacteria Isolated from Contaminated Mine Sediments. Applied and Environmental Microbiology, 81(24): 8478-8488. https://doi.org/10.1128/AEM.01970-15

Torma, A. E., Gabra, G. G, 1977. Oxidation of Stibnite by Thiobacillus Ferrooxidans. Antonie van Leeuwenhoek, 43(1): 1-6. https://doi.org/10.1007/BF02316204

Wang, N. N., Zhang, S. H., He, M. C, 2018. Bacterial Community Profile of Contaminated Soils in a Typical Antimony Mining Site. Environmental Science and Pollution Research, 25(1): 141-152. https://doi.org/10.1007/s11356-016-8159-y

Wang, Q., Garrity, G. M., Tiedje, J. M., et al., 2007. Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy. Mucosal Immunology, 73(16): 5261-5267. https://doi.org/10.1128/aem.00062-07

Xiang, L., Liu, C., Liu, D., et al., 2022. Antimony Transformation and Mobilization from Stibnite by an Antimonite Oxidizing Bacterium Bosea sp. AS-1. Journal of Environmental Sciences (China), 111: 273-281. https://doi.org/10.1016/j.jes.2021.03.042

Xiao, E. Z., Krumins, V., Dong, Y. R., et al., 2016. Microbial Diversity and Community Structure in an Antimony-Rich Tailings Dump. Applied Microbiology and Biotechnology, 100(17): 7751-7763. https://doi.org/10.1007/s00253-016-7598-1

Zhang, G. P., Ouyang, X. X., Li, H. X., et al., 2016. Bioremoval of Antimony from Contaminated Waters by a Mixed Batch Culture of Sulfate-Reducing Bacteria. International Biodeterioration and Biodegradation, 115: 148-155. https://doi.org/10.1016/j.ibiod.2016.08.007

江南, 李小倩, 周爱国, 等, 2020. pH值和氧化剂对硫化锑氧化溶解的影响机制. 地质科技通报, 39(4): 76-84. https://www.cnki.com.cn/Article/CJFDTOTAL-DZKQ202004010.htm

Relative Articles

Supplements(0)

Cited By

Proportional views