金矿化指示菌的分子生物学鉴定

杨娇艳; 张勇; 王红梅; 宋冬林; 程琴; 梅娟; 吴刚

Volume 31 Issue 3

May 2006

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Article Navigation > Earth Science > 2006 > 31(3): 355-360

YANG Jiao-yan, ZHANG Yong, WANG Hong-mei, SONG Dong-lin, CHENG Qin, MEI Juan, WU Gang, 2006. Molecular Identification of Gold-Mineralization Indicating Bacteria. Earth Science, 31(3): 355-360.

Citation:

YANG Jiao-yan, ZHANG Yong, WANG Hong-mei, SONG Dong-lin, CHENG Qin, MEI Juan, WU Gang, 2006. Molecular Identification of Gold-Mineralization Indicating Bacteria. Earth Science, 31(3): 355-360.

YANG Jiao-yan, ZHANG Yong, WANG Hong-mei, SONG Dong-lin, CHENG Qin, MEI Juan, WU Gang, 2006. Molecular Identification of Gold-Mineralization Indicating Bacteria. Earth Science, 31(3): 355-360.

Citation:

YANG Jiao-yan, ZHANG Yong, WANG Hong-mei, SONG Dong-lin, CHENG Qin, MEI Juan, WU Gang, 2006. Molecular Identification of Gold-Mineralization Indicating Bacteria. Earth Science, 31(3): 355-360.

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Molecular Identification of Gold-Mineralization Indicating Bacteria

1.
College of Life Science, Huazhong Normal University, Wuhan 430079, China
2.
Wuhan Institute of Viruology, Chinese Academy of Sciences, Wuhan 430071, China
3.
School of Environmental Studies, China University of Geosciences, Wuhan 430074, China

Received Date: 2005-04-05
Publish Date: 2006-05-25

Abstract

Abstract

Recently the microbial mineral exploration method, spore counting of Bacillus cereus in soil samples which is indicative for blind gold ore deposit, has been confirmed by many geologists in different countries. However, the identification of bacterial strains, indicators for gold mineralization, was still done through the culturing method in previous studies. This paper employed molecular biology technique to microbial identification in mineralized areas. Two bacterial strains as indicators of gold mineralization were randomly isolated from the gold deposit of Shewu mountain in Jiayu county, Hubei Province. On the basis of biochemical and physiological identification, phylogenetic analysis dedrived from 16S rDNA and 16S-23S rDNA sequence, strains Y1 and Y2 were identified as Bacillus mycoides and B. cereus respectively, both of which belong to "B. cereus group". So it is "B. cereus group" that is the indicator for gold mineralization instead of B. ceresus species. The results assured the reliability of the traditional selective culturing method of B. cereus group, and the feasibility of molecular biology techniques on mineral exploration. In addition, 16S rDNA and 16S-23S rDNA sequence analyses were helpful to make fluorogenic-probe reagent box in future work.
- 16SrDNA,
- 16S-23SrDNA internal transcribed spacer,
- bacteria identification,
- gold-mineralization indicating bacteria,
- microbial mineral exploration

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References

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