蓝细菌与蒙脱石对低温原白云石形成的协同促进效应

陈婷; 戴兆毅; 邱轩; 常标; 王红梅; 刘邓

doi:10.3799/dqkx.2025.200

Volume 50 Issue 12

Dec. 2025

Turn off MathJax

Article Contents

Article Navigation > Earth Science > 2025 > 50(12): 4938-4949

Chen Ting, Dai Zhaoyi, Qiu Xuan, Chang Biao, Wang Hongmei, Liu Deng, 2025. Synergistic Effects of Cyanobacteria and Montmorillonite on Formation of Low-Temperature Protodolomite. Earth Science, 50(12): 4938-4949. doi: 10.3799/dqkx.2025.200

Citation:

Chen Ting, Dai Zhaoyi, Qiu Xuan, Chang Biao, Wang Hongmei, Liu Deng, 2025. Synergistic Effects of Cyanobacteria and Montmorillonite on Formation of Low-Temperature Protodolomite. Earth Science, 50(12): 4938-4949. doi: 10.3799/dqkx.2025.200

Citation:

Chen Ting, Dai Zhaoyi, Qiu Xuan, Chang Biao, Wang Hongmei, Liu Deng, 2025. Synergistic Effects of Cyanobacteria and Montmorillonite on Formation of Low-Temperature Protodolomite. Earth Science, 50(12): 4938-4949. doi: 10.3799/dqkx.2025.200

PDF( 5362 KB)

Synergistic Effects of Cyanobacteria and Montmorillonite on Formation of Low-Temperature Protodolomite

doi: 10.3799/dqkx.2025.200

Chen Ting^{1, 2
,
,},
Dai Zhaoyi^{1, 2},
Qiu Xuan¹,
Chang Biao³,
Wang Hongmei^{1, 4},
Liu Deng^{1, 4
,
,
,}

1.
State Key Laboratory of Geomicrobiology and Environmental Changes, China University of Geosciences (Wuhan), Wuhan 430078, China
2.
School of Earth Sciences, China University of Geosciences (Wuhan), Wuhan 430074, China
3.
State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences (Wuhan), Wuhan 430078, China
4.
School of Environmental Studies, China University of Geosciences (Wuhan), Wuhan 430078, China

Received Date: 2025-08-15
Publish Date: 2025-12-25

Abstract

Abstract

The formation mechanism of dolomite [CaMg(CO₃)₂] remains a longstanding enigma in earth sciences. Previous studies have identified certain microorganisms and clay minerals as catalysts in the crystallization of low-temperature protodolomite, a crucial precursor to ordered dolomite. However, the role of cyanobacteria and particularly their potential synergistic effects with clay minerals remain poorly understood. In this study, we investigated bioprecipitation of carbonate minerals using the halotolerant cyanobacterium Synechococcus elongatus FACHB-410 in the presence and absence of montmorillonite. Our results demonstrated that protodolomite occurred as the predominant solid product in the montmorillonite-amended biosystems as confirmed by X-ray diffraction (XRD) and Raman spectroscopy, whereas monohydrocalcite and low-magnesian calcite were the primary products in the montmorillonite-free biosystems. Multiple microscopic techniques, including scanning electron microscopy (SEM), focused ion beam microscopy (FIB-SEM), and transmission electron microscopy (TEM), revealed that protodolomite nucleated as nanocrystals preferentially on montmorillonite surfaces. Density functional theory (DFT) simulations further elucidated that surface electronegativity of montmorillonite played a key role in promoting protodolomite formation by strongly adsorbing Mg²⁺ ions through electrostatic interactions, thereby facilitating their dehydration and significantly lowering the nucleation energy barrier.
- dolomite problem,
- clay minerals,
- protodolomite,
- cyanobacteria,
- microbial mineralization

FullText(HTML)

References(74)

References

Badger, M. R., Hanson, D., Price, G. D., 2002. Evolution and Diversity of CO₂ Concentrating Mechanisms in Cyanobacteria. Functional Plant Biology, 29(3): 161-173. https://doi.org/10.1071/PP01213

Bischoff, W. D., Bishop, F. C., Mackenzie, F. T., 1983. Biogenically Produced Magnesian Calcite; Inhomogeneities in Chemical and Physical Properties; Comparison with Synthetic Phases. American Mineralogist, 68(11-12): 1183-1188.

Bontognali, T. R. R., McKenzie, J. A., Warthmann, R. J., et al., 2014. Microbially Influenced Formation of Mg⁃Calcite and Ca⁃Dolomite in the Presence of Exopolymeric Substances Produced by Sulphate⁃Reducing Bacteria. Terra Nova, 26(1): 72-77. https://doi.org/10.1111/ter.12072

Bontognali, T. R. R., Vasconcelos, C., Warthmann, R. J., et al., 2010. Dolomite Formation within Microbial Mats in the Coastal Sabkha of Abu Dhabi (United Arab Emirates). Sedimentology, 57(3): 824-844. https://doi.org/10.1111/j.1365⁃3091.2009.01121.x

Brauchli, M., McKenzie, J. A., Strohmenger, C. J., et al., 2016. The Importance of Microbial Mats for Dolomite Formation in the Dohat Faishakh Sabkha, Qatar. Carbonates and Evaporites, 31(3): 339-345. https://doi.org/10.1007/s13146⁃015⁃0275⁃0

Chen, T., Qiu, X., Liu, D., et al., 2024. Dissolved Silicon as a Beneficial Factor for Biomineralization of Disordered Dolomite by a Halophilic Cyanobacterium. Chemical Geology, 670: 122435. https://doi.org/10.1016/j.chemgeo.2024.122435

Deng, S. C., Dong, H. L., Lyu, G., et al., 2010. Microbial Dolomite Precipitation Using Sulfate Reducing and Halophilic Bacteria: Results from Qinghai Lake, Tibetan Plateau, NW China. Chemical Geology, 278(3-4): 151-159. https://doi.org/10.1016/j.chemgeo.2010.09.008

Dong, H., Jaisi, D. P., Kim, J., et al., 2009. Microbe⁃Clay Mineral Interactions. American Mineralogist, 94(11-12): 1505-1519. https://doi.org/10.2138/am.2009.3246

Dong, H. L., Zeng, Q., Liu, D., et al., 2024. Interactions between Clay Minerals and Microbes: Mechanisms and Applications in Environmental Remediation. Earth Science Frontiers, 31(1): 467-485(in Chinese with English abstract).

Duan, Y., Yao, Y. C., Qiu, X., et al., 2017. Dolomite Formation Facilitated by Three Halophilic Archaea. Earth Science, 42(3): 389-396(in Chinese with English abstract).

Fan, Q. G., Liu, D., Papineau, D., et al., 2023. Precipitation of High Mg⁃Calcite and Protodolomite Using Dead Biomass of Aerobic Halophilic Bacteria. Journal of Earth Science, 34(2): 456-466. https://doi.org/10.1007/s12583⁃020⁃1108⁃1

Fang, Y. H., Hobbs, F., Yang, Y. P., et al., 2023. Dissolved Silica⁃Driven Dolomite Precipitation in the Great Salt Lake, Utah, and Its Implication for Dolomite Formation Environments. Sedimentology, 70(4): 1328-1347. https://doi.org/10.1111/sed.13081.

Görgen, S., Benzerara, K., Skouri⁃Panet, F., et al., 2020. The Diversity of Molecular Mechanisms of Carbonate Biomineralization by Bacteria. Discover Materials, 1(1): 2. https://doi.org/10.1007/s43939⁃020⁃00001⁃9

Gregg, J. M., Bish, D. L., Kaczmarek, S. E., et al., 2015. Mineralogy, Nucleation and Growth of Dolomite in the Laboratory and Sedimentary Environment: a Review. Sedimentology, 62(6): 1749-1769. https://doi.org/10.1111/sed.12202

Gunasekaran, S., Anbalagan, G., Pandi, S., 2006. Raman and Infrared Spectra of Carbonates of Calcite Structure. Journal of Raman Spectroscopy, 37(9): 892-899. https://doi.org/10.1002/jrs.1518

Huang, Y. R., Yao, Q. Z., Li, H., et al., 2019. Aerobically Incubated Bacterial Biomass⁃Promoted Formation of Disordered Dolomite and Implication for Dolomite Formation. Chemical Geology, 523: 19-30. https://doi.org/10.1016/j.chemgeo.2019.06.006

Kamennaya, N. A., Ajo⁃Franklin, C. M., Northen, T., et al., 2012. Cyanobacteria as Biocatalysts for Carbonate Mineralization. Minerals, 2(4): 338-364. https://doi.org/10.3390/min2040338

Kenward, P. A., Fowle, D. A., Goldstein, R. H., et al., 2013. Ordered Low⁃Temperature Dolomite Mediated by Carboxyl⁃Group Density of Microbial Cell Walls. AAPG Bulletin, 97(11): 2113-2125. https://doi.org/10.1306/05171312168

Kim, J., Kimura, Y., Puchala, B., et al., 2023. Dissolution Enables Dolomite Crystal Growth near Ambient Conditions. Science, 382(6673): 915-920. https://doi.org/10.1126/science.adi3690

Krause, S., Liebetrau, V., Gorb, S., et al., 2012. Microbial Nucleation of Mg⁃Rich Dolomite in Exopolymeric Substances under Anoxic Modern Seawater Salinity: New Insight into an Old Enigma. Geology, 40(7): 587-590. https://doi.org/10.1130/g32923.1

Land, L. S., 1998. Failure to Precipitate Dolomite at 25 ℃ from Dilute Solution Despite 1 000⁃Fold Oversaturation after 32 Years. Aquatic Geochemistry, 4(3): 361-368. https://doi.org/10.1023/A:1009688315854

Li, B., Yan, J. X., Liu, X. T., et al., 2010. The Organogenic Dolomite Model: Mechanism, Progress and Significance. Journal of Palaeogeography, 12(6): 699-710(in Chinese with English abstract).

Lippmann, F., 1973. Sedimentary Carbonate Minerals. Springer-Verlag Berlin, Heidelberg. https://doi.org/10.1007/978⁃3⁃642⁃65474⁃9

Liu, D., Fan, Q. G., Papineau, D., et al., 2020a. Precipitation of Protodolomite Facilitated by Sulfate⁃Reducing Bacteria: The Role of Capsule Extracellular Polymeric Substances. Chemical Geology, 533: 119415. https://doi.org/10.1016/j.chemgeo.2019.119415.

Liu, D., Xu, Y. Y., Yu, Q. Q., et al., 2020b. Catalytic Effect of Microbially⁃Derived Carboxylic Acids on the Precipitation of Mg⁃Calcite and Disordered Dolomite: Implications for Sedimentary Dolomite Formation. Journal of Asian Earth Sciences, 193: 104301. https://doi.org/10.1016/j.jseaes.2020.104301

Liu, D., Yu, N., Papineau, D., et al., 2019a. The Catalytic Role of Planktonic Aerobic Heterotrophic Bacteria in Protodolomite Formation: Results from Lake Jibuhulangtu Nuur, Inner Mongolia, China. Geochimica et Cosmochimica Acta, 263: 31-49. https://doi.org/10.1016/j.gca.2019.07.056

Liu, D., Xu, Y. Y., Papineau, D., et al., 2019b. Experimental Evidence for Abiotic Formation of Low⁃Temperature Proto⁃Dolomite Facilitated by Clay Minerals. Geochimica et Cosmochimica Acta, 247: 83-95. https://doi.org/10.1016/j.gca.2018.12.036.

McKenzie, J. A., Vasconcelos, C., 2009. Dolomite Mountains and the Origin of the Dolomite Rock of which they Mainly Consist: Historical Developments and New Perspectives. Sedimentology, 56(1): 205-219. https://doi.org/10.1111/j.1365⁃3091.2008.01027.x

Meister, P., Reyes, C., Beaumont, W., et al., 2011. Calcium and Magnesium⁃Limited Dolomite Precipitation at Deep Springs Lake, California. Sedimentology, 58(7): 1810-1830. https://doi.org/10.1111/j.1365⁃3091.2011.01240.x

Meng, R. R., Han, Z. Z., Gao, X., et al., 2024. Dissolved Ammonia Catalyzes Proto⁃Dolomite Precipitation at Earth Surface Temperature. Earth and Planetary Science Letters, 646: 119012. https://doi.org/10.1016/j.epsl.2024.119012

Netto, P. R. A., Pozo, M., da Silva, M. D., et al., 2022. Paleoenvironmental Implications of Authigenic Magnesian Clay Formation Sequences in the Barra Velha Formation (Santos Basin, Brazil). Minerals, 12(2): 200. https://doi.org/10.3390/min12020200

Obst, M., Dittrich, M., Kuehn, H., 2006. Calcium Adsorption and Changes of the Surface Microtopography of Cyanobacteria Studied by AFM, CFM, and TEM with Respect to Biogenic Calcite Nucleation. Geochemistry, Geophysics, Geosystems, 7(6): 2005GC001172. https://doi.org/10.1029/2005GC001172

Pérez, A. M., Zarza, A. M. A., La Iglesia, Á., et al., 2015. Do Magnesian Clays Play a Role in Dolomite Formation in Alkaline Environments? An Example from Castañar Cave, Cáceres (Spain). Geogaceta, 57: 15⁃18.

Perri, E., Tucker, M., 2007. Bacterial Fossils and Microbial Dolomite in Triassic Stromatolites. Geology, 35(3): 207. https://doi.org/10.1130/g23354a.1

Perri, E., Tucker, M. E., Mawson, M., 2013. Biotic and Abiotic Processes in the Formation and Diagenesis of Permian Dolomitic Stromatolites (Zechstein Group, NE England). Journal of Sedimentary Research, 83(10): 896-914. https://doi.org/10.2110/jsr.2013.65

Petrash, D. A., Bialik, O. M., Bontognali, T. R. R., et al., 2017. Microbially Catalyzed Dolomite Formation: From Near⁃Surface to Burial. Earth⁃Science Reviews, 171: 558-582. https://doi.org/10.1016/j.earscirev.2017.06.015

Qiu, X., Wang, H. M., Liu, D., et al., 2012. The Physiological Response of Synechococcus Elongatus to Salinity: A Potential Biomarker for Ancient Salinity in Evaporative Environments. Geomicrobiology Journal, 29(5): 477-483. https://doi.org/10.1080/01490451.2011.581331

Qiu, X., Wang, H. M., Yao, Y. C., et al., 2017. High Salinity Facilitates Dolomite Precipitation Mediated by Haloferax volcanii DS52. Earth and Planetary Science Letters, 472: 197-205. https://doi.org/10.1016/j.epsl.2017.05.018

Qiu, X., Yao, Y. C., Wang, H. M., et al., 2018. Live Microbial Cells Adsorb Mg²⁺ More Effectively than Lifeless Organic Matter. Frontiers of Earth Science, 12(1): 160-169. https://doi.org/10.1007/s11707⁃017⁃0626⁃3

Riding, R., 2006. Cyanobacterial Calcification, Carbon Dioxide Concentrating Mechanisms, and Proterozoic-Cambrian Changes in Atmospheric Composition. Geobiology, 4(4): 299-316. https://doi.org/10.1111/j.1472⁃4669.2006.00087.x

Ritz, M., Vaculíková, L., Kupková, J., et al., 2016. Different Level of Fluorescence in Raman Spectra of Montmorillonites. Vibrational Spectroscopy, 84: 7-15. https://doi.org/10.1016/j.vibspec.2016.02.007

Roberts, J. A., Bennett, P. C., González, L. A., et al., 2004. Microbial Precipitation of Dolomite in Methanogenic Groundwater. Geology, 32(4): 277. https://doi.org/10.1130/g20246.2

Rodriguez⁃Blanco, J. D., Shaw, S., Benning, L. G., 2015. A Route for the Direct Crystallization of Dolomite. American Mineralogist, 100(5-6): 1172-1181. https://doi.org/10.2138/am⁃2015⁃4963

Sánchez⁃Román, M., Romanek, C. S., Fernández⁃Remolar, D. C., et al., 2011. Aerobic Biomineralization of Mg⁃Rich Carbonates: Implications for Natural Environments. Chemical Geology, 281(3-4): 143-150. https://doi.org/10.1016/j.chemgeo.2010.11.020

Sánchez⁃Román, M., Vasconcelos, C., Schmid, T., et al., 2008. Aerobic Microbial Dolomite at the Nanometer Scale: Implications for the Geologic Record. Geology, 36(11): 879. https://doi.org/10.1130/g25013a.1

Sun, J. M., Wu, Z. G., Cheng, H. F., et al., 2014. A Raman Spectroscopic Comparison of Calcite and Dolomite. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 117: 158-162. https://doi.org/10.1016/j.saa.2013.08.014

Vasconcelos, C., McKenzie, J. A., Bernasconi, S., et al., 1995. Microbial Mediation as a Possible Mechanism for Natural Dolomite Formation at Low Temperatures. Nature, 377: 220-222. https://doi.org/10.1038/377220a0

Wanas, H. A., Sallam, E., 2016. Abiotically⁃Formed, Primary Dolomite in the Mid⁃Eocene Lacustrine Succession at Gebel El⁃Goza El⁃Hamra, NE Egypt: An Approach to the Role of Smectitic Clays. Sedimentary Geology, 343: 132-140. https://doi.org/10.1016/j.sedgeo.2016.08.003

Wang, X., Kong, X. X., Liu, Q., et al., 2023. Effect of Clay Minerals on Carbonate Precipitation Induced by Cyanobacterium Synechococcus Sp. Microbiology Spectrum, 11(3). https://doi.org/10.1128/spectrum.00363⁃23

Warren, J., 2000. Dolomite: Occurrence, Evolution and Economically Important Associations. Earth⁃Science Reviews, 52(1-3): 1-81. https://doi.org/10.1016/s0012⁃8252(00)00022⁃2

Xie, S. C., Yin, H. F., Liu, D., et al., 2018. On Development from Paleontology to Geobiology: Overview of Innovation and Expansion of Application Fields. Earth Science, 43(11): 3823-3836 (in Chinese with English abstract).

Xu, Y. Y., Liu, D., Yu, N., et al., 2018. Advance and Review on Microbial/Organogenic Dolomite Model. Earth Science, 43(Suppl. 1): 63-70 (in Chinese with English abstract).

Yao, T. T., Zhu, H. T., Yang, X. H., et al., 2020. Dolomite Origin of Shahejie Formation in Huanghekou Sag, Bohai Bay Basin. Earth Science, 45(10): 3567-3578 (in Chinese with English abstract).

Yao, Y. C., Qiu, X., Wang, H. M., et al., 2018. Dolomite Formation Mediated by Halophilic Archaeal Cells under Different Conditions and Carboxylated Microspheres. Earth Science, 43(2): 449-458 (in Chinese with English abstract).

You, X. L., Jia, W. Q., Xu, F., et al., 2018. Mineralogical Characteristics of Ankerite and Mechanisms of Primary and Secondary Origins. Earth Science, 43(11): 4046-4055 (in Chinese with English abstract).

You, X. L., Sun, S., Zhu, J. Q., et al., 2011. Progress in the Study of Microbial Dolomite Model. Earth Science Frontiers, 18(4): 52-64 (in Chinese with English abstract).

You, X. L., Sun, S., Zhu, J. Q., et al., 2013. Microbially Mediated Dolomite in Cambrian Stromatolites from the Tarim Basin, North⁃West China: Implications for the Role of Organic Substrate on Dolomite Precipitation. Terra Nova, 25(5): 387-395. https://doi.org/10.1111/ter.12048

Yu, N., Xu, Y. Y., Liu, D., et al., 2018. Catalytic Role of Anaerobic Bacteria in Dolomite Formation in Lake Jibuhulangtu Nuur, Inner Mongolia. Earth Science, 43(Suppl. 1): 53-62(in Chinese with English abstract).

Zhang, F. F., Xu, H. F., Konishi, H., et al., 2012. Dissolved Sulfide⁃Catalyzed Precipitation of Disordered Dolomite: Implications for the Formation Mechanism of Sedimentary Dolomite. Geochimica et Cosmochimica Acta, 97: 148-165. https://doi.org/10.1016/j.gca.2012.09.008

Zhang, F., Xu, H., Shelobolina, E. S., et al., 2015. The Catalytic Effect of Bound Extracellular Polymeric Substances Excreted by Anaerobic Microorganisms on Ca⁃Mg Carbonate Precipitation: Implications for the "Dolomite Problem". American Mineralogist, 100(2/3): 483-494. https://doi.org/10.2138/am⁃2015⁃4999

Zhao, S. B., Liu, Y. C., Yue, L. L., et al., 2025. Types, Characteristics, and Genesis of Lower Carboniferous Baizuo Formation Dolomite in Super⁃Large Huize Pb⁃Zn Orefield. Earth Science, 50(4): 1353-1379 (in Chinese with English abstract).

Zhao, Y. Y., Wei, X. Y., Gao, X., et al., 2024. Proto⁃Dolomite Spherulites with Heterogeneous Interior Precipitated in Brackish Water Cultivation of Freshwater Cyanobacterium Leptolyngbya boryana. Science of the Total Environment, 906: 167552. https://doi.org/10.1016/j.scitotenv.2023.167552

Zheng, W. L., Liu, D., Yang, S. S., et al., 2021. Transformation of Protodolomite to Dolomite Proceeds under Dry⁃Heating Conditions. Earth and Planetary Science Letters, 576: 117249. https://doi.org/10.1016/j.epsl.2021.117249

董海良, 曾强, 刘邓, 等, 2024. 黏土矿物-微生物相互作用机理以及在环境领域中的应用. 地学前缘, 31(1): 467-485.

段勇, 药彦辰, 邱轩, 等, 2017. 三株嗜盐古菌诱导形成白云石. 地球科学, 42(3): 389-396.

李波, 颜佳新, 刘喜停, 等, 2010. 白云岩有机成因模式: 机制、进展与意义. 古地理学报, 12(6): 699-710.

谢树成, 殷鸿福, 刘邓, 等, 2018. 再谈古生物学向地球生物学的发展: 服务领域的拓展与创新. 地球科学, 43(11): 3823-3836. doi: 10.3799/dqkx.2018.169

许杨阳, 刘邓, 于娜, 等, 2018. 微生物(有机)白云石成因模式研究进展与思考. 地球科学, 43(增刊1): 63-70. doi: 10.3799/dqkx.2018.513

姚婷婷, 朱红涛, 杨香华, 等, 2020. 渤海湾盆地黄河口凹陷沙河街组白云岩成因机理. 地球科学, 45(10): 3567-3578. doi: 10.3799/dqkx.2020.227

药彦辰, 邱轩, 王红梅, 等, 2018. 不同状态嗜盐古菌细胞及羧基微球诱导白云石沉淀. 地球科学, 43(2): 449-458. doi: 10.3799/dqkx.2017.579

由雪莲, 孙枢, 朱井泉, 等, 2011. 微生物白云岩模式研究进展. 地学前缘, 18(4): 52-64. doi: 10.3799/dqkx.2018.513

由雪莲, 贾文强, 徐帆, 等, 2018. 铁白云石矿物学特征及原生次生成因机制. 地球科学, 43(11): 4046-4055. doi: 10.3799/dqkx.2018.152

于娜, 许杨阳, 刘邓, 等, 2018. 内蒙古吉布胡郎图诺尔盐湖厌氧菌对白云石形成的催化作用. 地球科学,43(增刊 1): 53-62. doi: 10.3799/dqkx.2018.543

赵思博, 刘英超, 岳龙龙, 等, 2025. 会泽铅锌矿区摆佐组地层白云石类型、特征及成因. 地球科学, 50(4): 1353-1379. doi: 10.3799/dqkx.2024.076

Relative Articles

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Figures(8) / Tables(1)

Get Citation

PDF

XML

Article views (159) PDF downloads(8)

Synergistic Effects of Cyanobacteria and Montmorillonite on Formation of Low-Temperature Protodolomite

doi: 10.3799/dqkx.2025.200

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Proportional views

Export File

Citation

Format

Content