仰韶文化人类遗骸古DNA的初步研究

赖旭龙; 杨淑娟; 唐先华; 施苏华; 李润权; 杨洪; 高强; 李涛; 盛桂莲

Volume 29 Issue 1

Jan. 2004

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Article Navigation > Earth Science > 2004 > 29(1): 15-20

LAI Xu-long, YANG Shu-juan, TANG Xian-hua, SHI Su-hua, LI Run-quan, YANG Hong, GAO Qiang, LI Tao, SHENG Gui-lian, 2004. Preliminary Study on Ancient Human DNA from Yangshao Culture. Earth Science, 29(1): 15-20.

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Preliminary Study on Ancient Human DNA from Yangshao Culture

1.
Faculty of Earth Sciences, China University of Geosciences, Wuhan 430074, China
2.
College of Life Sciences, Zhongshan University, Guangzhou 510275, China
3.
Department of Anthropology, Harvard University, Cambridge, MA 02138-2019, USA
4.
Department of Science and Tech nology, Bryant College, Smithfield, RI 02917 and Department of Geological Sciences, Brown University, Providence, RI 02912, USA
5.
Xi'an Banpo Site Museum, Xi'an 710038, China

Received Date: 2003-09-02
Publish Date: 2004-01-25

Abstract

Abstract

During recent 20 years, the field of ancient DNA research has experienced a rapid development. Authentic DNA sequences from ancient human remains have provided very important information on human evolution, blood relationship and migration, making ancient DNA research an important field of molecular anthropology. This study illustrates ancient DNA extraction, amplification and sequencing of 6 000-year-B.P. Yangshao Culture ancient human remains collected from first and second phase culture beds at Jiangzhai Site, Lintong, Shaanxi Province. Three 169 bp fragments from the hypervariable region Ⅰ in the mitochondrial genome have been obtained and authenticated. These ancient DNA sequences carry two point mutations in comparison with homologeous sequence of the modern Xi'an people's. Moreover, the authenticity of the ancient DNA sequence was obtained through the replication experiments in two different laboratories in China University of Geosciences and Zhongshan University as well as phylogenetic analysis. Among the 6 samples from the Jiangzhai Site, three of them yielded authenticated ancient DNA sequence. The 50% success ratio is higher than that of general ancient DNA research materials previously reported in the literature, indicating the potential for further ancient DNA studies on human remains of Yangshao Culture for the purpose of blood relationship among different archaeological sites and human evolution.
- archaeology,
- Yangshao Culture,
- Jiangzhai Site,
- ancient DNA,
- authenticity analysis

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References

Cui, Y. Q., Duan, R. H., Ji, C. N., etal., 2002. Analysis of mi- tochondrial from ancient ruins of Jiaohe. Chemical Journal of Chinese Universities, 8: 1510-1514 (in Chinese with English abstract).

Gao, Q., Lee, Y. K., 1993. A biological perspective on Yangshao Kinship. Journal of Anthropological Archaeology, 12 (3): 226-298.

Goldberg, T. L., Ruvolo, M., 1997. Molecular phylogenetics and historical biogeography of East African chimpanzees pan troglodytes schweinfurthii. Biol. J. Linn. Soc. Lond., 61 (3): 301-324. doi: 10.1111/j.1095-8312.1997.tb01794.x

Herrmann, B., Hummel, S., 1994. Ancient DNA. Springer-Ver-lag, New York.

HÖss, M., Jaruga, P., Zastawnj, T. H., etal., 1996. DNA dam-age and DNA sequence retrieval from ancient tissues. Nu-cleic Acids Research, 24: 1304-1307. doi: 10.1093/nar/24.7.1304

Institute of Archaeology, 1991. δ¹⁴C dating data (1965—1991) of Chinese archaeology. Wenwu Press, Beijing, 262-263 (in Chinese).

Kolman, C. J., Sambuughin, N., Bermingham, E., 1996. Mito-chondrial DNA analysis of Mongolian populations and im-plications for the origin of New World founders. Genetics, 142 (4): 1321-1334. doi: 10.1093/genetics/142.4.1321

Krings, M., Stone, A., Schmitz, R. W., et al., 1997. Neanderthal DNA sequences and the origin of modern humans. Cell, 90 (1): 19-30. doi: 10.1016/S0092-8674(00)80310-4

Lai, X. L., 2001. Ancient biomolecules and molecular archaeolo-gy—A review. Advance in Earth Sciences, 16 (2): 163-171 (in Chinese with English abstract).

Lin, Q., Shi, S. H., Peng, P. A., et al., 2002. DNA extracted from plant materials buried inperennial frozen sediments. Journal of Glaciology and Geocryology, 24 (1): 28-31 (in Chinese with English abstract).

Lindahl, T., 1997. Facts and artifacts of ancient DNA. Cell, 90: 1-3. doi: 10.1016/S0092-8674(00)80306-2

Macaulay, V., Richards, M., Hickey, E., et al., 1999. Thee merging tree of West Eurasian mtDNAs: A synthesis of con-trol -region sequences and RFLPs. Am. J. Hum. Genet., 64 (1): 232-249. doi: 10.1086/302204

Oota, H., Kitano, T., Jin, F., et al., 2002. Extreme mtDNA homogeneity in continental Asian populations. Am. J. Phys. Anthropol, 118 (2): 146-153. doi: 10.1002/ajpa.10056

Ovchinnikov, I. V., Götherström, A., Romanova, G. P., 2000. Molecular analysis of Neandertal DNA from the northern Caucasus. Nature, 404: 490-493. doi: 10.1038/35006625

Poinar, H. N., 1999. DNA from fossils: The past and the future. Acta Paediatrica, 88: 133-140. doi: 10.1111/j.1651-2227.1999.tb14423.x

Poinar, H. N., 2003. The top 10 list: Criteria of authenticity for DNA from ancient and forensic samples. International Con-gress Series, 1239: 575-579. doi: 10.1016/S0531-5131(02)00624-6

Poinar, H. N., Höss, M., Bada, J. L., et al., 1996. Amino acid racemization and the preservation of ancient DNA. Science, 272: 864-866. doi: 10.1126/science.272.5263.864

Thomas, W. K., Pääbo, S., Villablanca, F. X., et al., 1990. Spa-tial and temporal continuity of kangaroo rat populations shown by sequencing mitochondrial DNA from museum specimens. J. Mol. Evol., 31: 101-112. doi: 10.1007/BF02109479

Wan, C., Cui, Y. Q., Duan, R. H., et al., 2001. Extraction, am-plification and sequence analysis of Xiajiadian ancient hu-man bone DNA. Chinese Journal of Biochemistry and Mo-lecular Biology, 17 (5): 636-641 (in Chinese with English abstract).

Wang, L., Oota, H., Saitou, N., et al., 2000. Genetic structure of a 2 500-year-old human population in China and its spa-tiotemporal changes. Mol. Biol. Evol., 17 (9): 1396-1400. doi: 10.1093/oxfordjournals.molbev.a026422

Watson, E., Bauer, K., Aman, R., et al., 1996. mtDNA se-quence diversity in Africa. Am. J. Hum. Genet., 59 (2): 437-444.

Wayne, R. K., Leonard, J. A., Cooper, A., 1999. Full of sound and fury: The recent history of ancient DNA. Annu. Rev. Ecol. Syst., 30: 457-477. doi: 10.1146/annurev.ecolsys.30.1.457

Xi'an Banpo Site Museum, Shaanxi Provincial Archaeology Insti-tute, Lintong County Museum, 1988. Excavation report of Jiangzhai—A Neolithic archaeological site. Wenwu Press, Beijing (in Chinese).

Yang, H., 1995. Authentication of ancient DNA sequence—A reassessment of 18S rDNA sequence from fossil dinosaur egg. Acta Palaeontologica Sinica, 34 (6): 657-673 (in Chinese with English abstract).

Yang, H., Golenberg, E. M., Shoshani, J. A., 1997. Blind tes-ting design for authenticating ancient DNA sequence. Mo-lecular Phylogenetics and Evolution, 7: 261-265. doi: 10.1006/mpev.1996.0398

Yang, Q., Ozawa, T., Hayashi, S., et al., 1999. Extraction, PCR amplification and cloning of aDNA from human remains of the warring states (475B. C-221B. C). Palaeoworld, 12: 12-24.

Yang, S. J., Lai, X. L., 2003. The first case of Mammoth an cient DNA in China. Earth Science—Journal of China University of Geosciences, 28 (2): 136, 142 (in Chinese with English abstract).

崔银秋, 段然慧, 季朝能, 等, 2002. 交河故城车师人的线粒体DNA分析. 高等学校化学学报, 8: 1510-1514. doi: 10.3321/j.issn:0251-0790.2002.08.010

中国社会科学院考古所, 1991. 中国考古学中碳十四年代数据1965—1991. 北京: 文物出版社, 262-263. https://www.cnki.com.cn/Article/CJFDTOTAL-KAGU198403015.htm

赖旭龙, 2001. 古代生物分子与分子考古学. 地球科学进展, 16 (2): 163-171. doi: 10.3321/j.issn:1001-8166.2001.02.004

林清, 施苏华, 彭平安, 等, 2002. 青藏高原多年冻土沉积物中埋藏植物DNA特征的初步分析. 冰川冻土, 24 (1): 28-31. doi: 10.3969/j.issn.1000-0240.2002.01.004

万诚, 崔银秋, 段然慧, 等, 2001. 夏家店等古人骨DNA的提取、扩增及序列分析. 中国生物化学与分子生物学报, 17 (5): 636-641. doi: 10.3969/j.issn.1007-7626.2001.05.018

西安半坡博物馆, 陕西省考古研究所, 临潼县博物馆, 1988. 姜寨-新石器时代遗址发掘报告. 北京: 文物出版社.

杨洪, 1995. 古代DNA序列的分析与甄别———兼评恐龙DNA研究. 古生物学报, 34 (6): 657-673. https://www.cnki.com.cn/Article/CJFDTOTAL-GSWX506.000.htm

杨淑娟, 赖旭龙, 2003. 中国第一例猛犸象古DNA研究. 地球科学———中国地质大学学报, 28 (2): 136, 142.

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