铁还原菌Shewanella oneidensis MR-1在H2O2扰动下的响应及机制

赵雨溪; 孙群群; 童曼; 袁松虎

doi:10.3799/dqkx.2022.115

Volume 48 Issue 4

Apr. 2023

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Article Navigation > Earth Science > 2023 > 48(4): 1649-1656

Zhao Yuxi, Sun Qunqun, Tong Man, Yuan Songhu, 2023. Response and Mechanism of Iron-Reducing Bacterium Shewanella oneidensis MR-1 to Perturbance of H2O2. Earth Science, 48(4): 1649-1656. doi: 10.3799/dqkx.2022.115

Citation:

Zhao Yuxi, Sun Qunqun, Tong Man, Yuan Songhu, 2023. Response and Mechanism of Iron-Reducing Bacterium Shewanella oneidensis MR-1 to Perturbance of H₂O₂. Earth Science, 48(4): 1649-1656. doi: 10.3799/dqkx.2022.115

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Response and Mechanism of Iron-Reducing Bacterium Shewanella oneidensis MR-1 to Perturbance of H₂O₂

doi: 10.3799/dqkx.2022.115

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430078, China

Received Date: 2021-12-30
Publish Date: 2023-04-25

Abstract

Abstract

Iron cycling mediated by iron-reducing bacteria is an important factor driving material cycle in the surface system of earth. H₂O₂ naturally generated and artificially injected into the subsurface environment may affect the activity and function of iron-reducing bacteria through oxidative stress, but the response and mechanism of iron-reducing bacteria to H₂O₂ disturbance is still unclear. In this study, Shewanella oneidensis MR-1 was chosen as a representative iron-reducing bacterium. In combination with batch experiments and RNA-seq analysis, the changes of MR-1 activity and function under different concentrations of H₂O₂ and its regulatory mechanism were investigated. Results show that MR-1 could resist H₂O₂ stress effectively, and H₂O₂ enhanced the Fe(Ⅲ)-reducing ability of MR-1. RNA-seq results show that MR-1 maintained in an anti-stress state infacing to H₂O₂ disturbance, which could resist the negative effects of H₂O₂ by actively oxidizing organic matter to provide energy and promoting the synthesis of catalase.
- iron cycling,
- iron-reducing bacteria,
- oxidative stress,
- geological microorganism,
- environmental geology

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Response and Mechanism of Iron-Reducing Bacterium Shewanella oneidensis MR-1 to Perturbance of H2O2

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Response and Mechanism of Iron-Reducing Bacterium Shewanella oneidensis MR-1 to Perturbance of H₂O₂