Abstract: Iron geochemistry has rapidly attracted great interest for global change research over the last two decades due to its significant role in the evolution of earth environment and in marine primary productivity. The speciation of particulate iron is critical for better understanding of iron transformation, transitivity and bioavailability. This paper systematically reviews the analytical development of lowtemperature particulate Fe speciation, including the degree of pyritization (DOP), Tessiers sequential extraction method, and specific iron extraction procedure. The application of threestep iron extraction is introduced, and some suggestions are given for the future iron speciation analysis based on the latest development of iron geochemical study