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    铁还原菌Shewanella oneidensis MR-1在H2O2扰动下的响应及机制

    赵雨溪 孙群群 童曼 袁松虎

    赵雨溪, 孙群群, 童曼, 袁松虎, 2023. 铁还原菌Shewanella oneidensis MR-1在H2O2扰动下的响应及机制. 地球科学, 48(4): 1649-1656. doi: 10.3799/dqkx.2022.115
    引用本文: 赵雨溪, 孙群群, 童曼, 袁松虎, 2023. 铁还原菌Shewanella oneidensis MR-1在H2O2扰动下的响应及机制. 地球科学, 48(4): 1649-1656. doi: 10.3799/dqkx.2022.115
    Zhao Yuxi, Sun Qunqun, Tong Man, Yuan Songhu, 2023. Response and Mechanism of Iron-Reducing Bacterium Shewanella oneidensis MR-1 to Perturbance of H2O2. Earth Science, 48(4): 1649-1656. doi: 10.3799/dqkx.2022.115
    Citation: Zhao Yuxi, Sun Qunqun, Tong Man, Yuan Songhu, 2023. Response and Mechanism of Iron-Reducing Bacterium Shewanella oneidensis MR-1 to Perturbance of H2O2. Earth Science, 48(4): 1649-1656. doi: 10.3799/dqkx.2022.115

    铁还原菌Shewanella oneidensis MR-1在H2O2扰动下的响应及机制

    doi: 10.3799/dqkx.2022.115
    基金项目: 

    国家自然科学基金项目 41703113

    国家自然科学基金项目 42025703

    详细信息
      作者简介:

      赵雨溪(1997—),女,硕士研究生,主要从事锰生物氧化研究. ORCID:0000-0002-7801-1602. E-mail:824813080@qq.com

      通讯作者:

      童曼,ORCID:0000-0002-0286-0608. E-mail:tongman@cug.edu.cn

    • 中图分类号: P69

    Response and Mechanism of Iron-Reducing Bacterium Shewanella oneidensis MR-1 to Perturbance of H2O2

    • 摘要: 铁还原菌参与的铁循环是地表系统中物质循环的重要驱动力,自然生成和人工注入地下环境的H2O2可能通过氧化胁迫影响铁还原菌的活性和功能,但铁还原菌在H2O2扰动下的响应及机制仍不清楚.以铁还原模式菌株Shewanella oneidensis MR-1作为研究对象,结合批实验和转录组测序研究了不同浓度水平H2O2扰动下MR-1活性和功能的变化及其调控机制.结果表明,MR-1能够有效抵御H2O2的胁迫,且H2O2扰动使MR-1的铁还原能力增强.转录组测序表明,H2O2使MR-1处于抗应激状态,通过积极氧化有机物供能、促进过氧化氢酶的合成抵御H2O2的负面影响.

       

    • 图  1  不同浓度H2O2扰动下MR-1的生长曲线

      Fig.  1.  Effects of different concentrations of H2O2 on the viability of MR-1

      图  2  有菌和无菌体系中H2O2浓度变化

      Fig.  2.  Variations of H2O2 concentration in the presence and absence of MR-1

      图  3  H2O2对CAT活性的影响

      Fig.  3.  Effect of H2O2 on the activity of CAT

      图  4  H2O2对MR-1铁还原能力的影响

      与空白组相比,***P < 0.001,****P < 0.000 1

      Fig.  4.  Effect of H2O2 on Fe(Ⅲ) reduction capacity of MR-1

      图  5  关键差异基因表达火山图

      Fig.  5.  Volcano plot of key differential gene expression

      表  1  差异基因表达情况

      Table  1.   Differential gene expression results

      基因ID log2(变化倍数) P值 描述
      SO_RS10090 -5.67 4.44×10-93 海洋变形菌分选酶靶蛋白
      SO_RS10085 -6.46 7.06×10-81 分选酶相关ompa样蛋白PdsO
      SO_RS10095 -3.96 1.36×10-58 GN分选酶
      SO_RS03930 -3.77 8.98×10-54 OXA-48家族水解酶
      SO_RS06920 3.39 1.25×10-45 苏氨酸脱氢酶基因
      SO_RS07720 3.10 7.72×10-37 外膜蛋白OmpW
      SO_RS15335 3.00 1.64×10-35 细胞色素d泛醇氧化酶亚基Ⅱ
      SO_RS17985 3.92 6.21×10-33 钼酸盐ABC转运蛋白底物结合蛋白
      SO_RS15340 2.82 6.21×10-33 细胞色素泛醇氧化酶亚基Ⅰ
      SO_RS10080 -3.28 4.57×10-28 变形菌分选酶系统反应调节器
      SO_RS18135 2.50 8.75×10-28 孔蛋白
      SO_RS13090 2.25 1.56×10-18 碳饥饿蛋白A
      SO_RS06640 2.20 1.34×10-17 钼喋呤支撑的氧化还原酶
      SO_RS09405 1.97 2.17×10-17 蛋白-蛋氨酸-亚砜还原酶催化亚基MsrP
      SO_RS01505 3.27 6.67×10-17 鸟氨酸脱羧酶
      SO_RS14360 2.08 2.56×10-15 Sigma70家族RNA聚合酶因子
      SO_RS21580 2.04 2.98×10-15 H家族蛋白
      SO_RS19750 -2.79 4.06×10-15 ISSod4家族转座酶
      SO_RS03335 1.89 4.38×10-15 分子伴侣GroEL
      SO_RS10010 2.51 5.15×10-15 细胞色素C氧化酶
      SO_RS10075 -2.83 1.57×10-14 变形杆菌专用分选酶系统组氨酸激酶
      SO_RS20140 -2.92 2.08×10-14 细胞包膜完整性蛋白CreD
      SO_RS04535 -1.76 2.08×10-14 ISSod2家族转座酶
      SO_RS09350 -1.76 2.08×10-14 ISSod2家族转座酶
      SO_RS09940 -1.76 2.08×10-14 ISSod2家族转座酶
      SO_RS19795 -1.76 2.08×10-14 ISSod2家族转座酶
      SO_RS21600 1.75 1.49×10-12 含有DUF3300结构域的蛋白
      SO_RS08155 1.63 3.25×10-12 OmcA/MtrC家族血红素c型细胞色素
      SO_RS14365 1.80 6.92×10-12 含有DUF3379结构域的蛋白
      SO_RS07745 1.70 1.60×10-11 辅酶a酰化甲基丙二酸半醛脱氢酶
      下载: 导出CSV

      表  2  GO富集分析结果

      Table  2.   GO enrichment analysis results

      GO ID 描述 P 分类 差异基因数量
      GO: 1901606 α-氨基酸分解代谢过程 6.13×10-5 BP 8
      GO: 0009063 细胞氨基酸分解代谢过程 1.18×10-4 BP 8
      GO: 0016054 有机酸分解代谢过程 1.11×10-3 BP 8
      GO: 0046395 羧酸分解代谢过程 1.11×10-3 BP 8
      GO: 0055114 氧化还原过程 3.00×10-3 BP 25
      GO: 0006091 前体代谢产物和能量的产生 3.36×10-3 BP 14
      GO: 0044282 小分子分解代谢过程 3.51×10-3 BP 8
      GO: 1901565 有机氮化合物分解代谢过程 3.56×10-3 BP 8
      GO: 0016491 氧化还原酶 8.02×10-3 MF 24
      GO: 0006536 谷氨酸代谢过程 1.15×10-2 BP 4
      GO: 0009056 分解代谢过程 1.27×10-2 BP 11
      GO: 0044248 细胞分解代谢过程 1.94×10-2 BP 9
      GO: 0022900 电子传递链 1.94×10-2 BP 10
      GO: 0006574 缬氨酸分解代谢过程 2.60×10-2 BP 2
      GO: 1901575 有机物分解代谢过程 2.60×10-2 BP 10
      GO: 0015980 由有机化合物氧化产生的能量 2.74×10-2 BP 6
      GO: 0045333 细胞呼吸 3.34×10-2 BP 5
      GO: 0009259 核糖核苷酸代谢过程 3.34×10-2 BP 7
      GO: 0009156 磷酸核糖核苷生物合成过程 3.34×10-2 BP 5
      GO: 1901564 有机氮化合物代谢过程 3.34×10-2 BP 33
      下载: 导出CSV
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    • 收稿日期:  2021-12-30
    • 刊出日期:  2023-04-25

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