锰离子对微生物诱导白云石沉淀的影响

doi:10.3799/dqkx.2026.179

锰离子对微生物诱导白云石沉淀的影响

doi: 10.3799/dqkx.2026.179

1 中国地质大学(武汉）地质微生物与环境全国重点实验室, 环境学院, 430078;
2 中国地质大学(武汉）未来技术学院, 430074

基金项目:

国家自然基金委重点基金项目资助(42330201)。

详细信息

作者简介:
蔡新宇(2000-),环境科学与工程硕士研究生,主要从事微生物-矿物相互作用的研究。ORCID:0009-0005-6771-0431,E-mail:414446894@qq.com

通讯作者:
王红梅,ORCID:0000-0001-7621-7810,E-mail:wanghmei04@163.com。研究工作受到国家自然基金委重点基金项目资助(42330201)

中图分类号: P571
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出版历程
- 收稿日期: 2026-04-30
- 网络出版日期: 2026-06-29

Influence of Mn²⁺ on Microbe-Induced Protodolomite Formation

1 State Key Laboratory of Geomicrobiology and Environmental Changes, School of the Environmental Studies, China University of Geosciences, Wuhan, 430078;
2 School of Future Technology, China University of Geosciences, Wuhan, 430074

摘要

摘要: “微生物白云石模式”为解释“白云石之谜”提供了思路，但多数实验缺乏微量元素输入。本研究分析不同浓度Mn²⁺（0、0.6、3、6 mmol/L）对细菌Vreelandella alkaliphila J1诱导原白云石沉淀的影响。通过水化学监测、胞外聚合物（EPS）组分分析、X射线衍射（XRD）及扫描电镜-元素能谱（SEM-EDS）等表征手段，揭示Mn²⁺通过影响微生物EPS组分调控原白云石沉淀的机制。结果发现低浓度Mn²⁺（0.6 mmol/L）促使细胞产生富含羧基的蛋白质，使矿物晶格Mg/Ca增加，有利于原白云石形成。高浓度Mn²⁺（3-6 mmol/L）抑制微生物生长及EPS分泌，Mn²⁺更易与CO₃^2-结合形成菱锰矿，从而抑制原白云石形成。本结果丰富对微生物白云石成因机制的认识，揭示了Mn抑制原白云石形成的浓度效应，对理解地质记录中富Mn白云岩的形成有一定的启示作用。
- 微量元素 /
- 生物矿化 /
- 原白云石 /
- 胞外聚合物(EPS) /
- Vreelandella alkaliphila J1
Abstract: Although the “microbial dolomite model” provides insights into explaining the “dolomite problem”, most of the experiments lack the input of trace elements. Therefore, the study investigated the influence of different concentrations of Mn²⁺(0, 0.6, 3, 6 mmol/L) on the protodolomite precipitation induced by the bacterial strain Vreelandella alkaliphila J1. By monitoring hydrochemical conditions and analyzing extracellular polymeric substances (EPS) components, combined with XRD and SEM-EDS, this study revealed the mechanism by which Mn²⁺ regulates protodolomite precipitation by influencing the composition of microbial EPS. The results showed that low-concentration Mn²⁺ (0.6 mmol/L) promoted the secretion of proteins rich in carboxyl groups and increased the Mg/Ca ratio in the mineral lattice, thereby facilitating protodolomite formation. In contrast, high-concentration Mn²⁺ (3-6 mmol/L) suppressed bacterial growth and EPS secretion. At this point, Mn²⁺ preferred to combine with CO₃^2- to form rhodochrosite, thereby inhibiting the formation of protodolomite. These findings confirmed that low concentrations of Mn²⁺ promoted the formation of protodolomite by enhancing EPS secretion, while high concentrations of Mn²⁺ inhibited its formation by inhibiting microbial growth and binding CO₃^2-. These findings improve our understanding of the mechanism underlying microbial protodolomite formation under the influence of Mn²⁺ and clarify the concentration-dependent effect of Mn²⁺ on protodolomite formation inhibition. Furthermore, this work provides implications for interpreting of Mn-rich dolomite in geological records.
- Trace element /
- Biomineralization /
- Protodolomite /
- Extracellular polymeric substances(EPS) /
- Vreelandella alkaliphila J1

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参考文献(40)

[1]	Bibi, I., Amin, A., Haese, R.R., Niazi, N.K., 2026. Unraveling Dolomite Dissolution Stoichiometry in Circumneutral to Alkaline pH Environments.Acta Geochimica45 (1), 132-141. https://doi.org/10.1007/s11631-025-00758-x
[2]	Daye, M., Higgins, J., Bosak, T., 2019. Formation of Ordered Dolomite in Anaerobic Photosynthetic Biofilms.Geology47 (6), 509-512. https://doi.org/10.1130/G45821.1
[3]	Diloreto, Z.A., Garg, S., Bontognali, T.R.R., Dittrich, M., 2021. Modern Dolomite Formation Caused by Seasonal Cycling of Oxygenic Phototrophs and Anoxygenic Phototrophs in a Hypersaline Sabkha.Scientific Reports11 (1), 4170. https://doi.org/10.1038/s41598-021-83676-1
[4]	Fang, Y., Xu, H., 2022. Dissolved Silica-Catalyzed Disordered Dolomite Precipitation.American Mineralogist107 (3), 443-452. https://doi.org/10.2138/am-2021-7474
[5]	Fang, Y., Xu, H., 2018. Study of an Ordovician Carbonate with Alternating Dolomite-Calcite Laminations and Its Implication for Catalytic Effects of Microbes on the Formation of Sedimentary Dolomite.Journal of Sedimentary Research88 (6), 679-695. https://doi.org/10.2110/jsr.2018.35
[6]	Geske, A., Zorlu, J., Richter, D.K., Buhl, D., Niedermayr, A., Immenhauser, A., 2012. Impact of Diagenesis and Low Grade Metamorphosis on Isotope (δ26Mg, δ13C, δ18O and 87Sr/86Sr) and Elemental (Ca, Mg, Mn, Fe and Sr) Signatures of Triassic Sabkha Dolomites.Chemical Geology332-333, 45-64. https://doi.org/10.1016/j.chemgeo.2012.09.014
[7]	Goldsmith, J.R., Graf, D.L., Heard, H.C., 1961. Lattice Constants of the Calcium Magnesium Carbonates.American Mineralogist46 (3-4), 453-459.
[8]	Gregg, J.M., Bish, D.L., Kaczmarek, S.E., Machel, H.G., 2015. Mineralogy, Nucleation and Growth of Dolomite in the Laboratory and Sedimentary Environment: A Review.Sedimentology62 (6), 1749-1769. https://doi.org/10.1111/sed.12202
[9]	Guo, C., Chen, D.Z., Fu, Y., Zhou, X., Liu, C., 2025. Origin of Burrow-Associated Dolomites and Its Reservoir Implications: A Case Study from the Lower-Middle Ordovician Carbonates of Tarim Basin (NW China).Journal of Earth Science36 (4), 1568-1590. https://doi.org/10.1007/s12583-022-1673-6
[10]	Han, Z., Li, J., Zhao, Y., Chen, Q., Gao, X., Hu, K., Guo, N., Wei, X., Meng, R., Zhu, C., Tucker, M.E., Han, C., 2024. Dissolved Mn2+Promotes Microbially-Catalyzed Protodolomite Precipitation in Brackish Oxidized Water.Chemical Geology650, 121986. https://doi.org/10.1016/j.chemgeo.2024.121986
[11]	Hobbs, F.W.C., Fang, Y., Lebrun, N., Yang, Y., Xu, H., 2024. Co-Precipitation of Primary Dolomite and Mg-Rich Clays in Deep Springs Lake, California.Sedimentology71 (4), 1363-1383. https://doi.org/10.1111/sed.13176
[12]	Krause, S., Liebetrau, V., Gorb, S., Sánchez-Román, M., McKenzie, J.A., Treude, T., 2012. Microbial Nucleation of Mg-Rich Dolomite in Exopolymeric Substances under Anoxic Modern Seawater Salinity: New Insight into an Old Enigma.Geology40 (7), 587-590. https://doi.org/10.1130/G32923.1
[13]	Land, L.S., 1998. Failure to Precipitate Dolomite at 25 ℃ from Dilute Solution Despite 1000-Fold Oversaturation after 32 Years.Aquatic Geochemistry4 (3-4), 361-368. https://doi.org/10.1023/A:1009688315854
[14]	Li, Q., Zhang, Y., Dong, L., Guo, Z., 2018. Oligocene Syndepositional Lacustrine Dolomite: A Study from the Southern Junggar Basin, NW China.Palaeogeography, Palaeoclimatology, Palaeoecology503, 69-80. https://doi.org/10.1016/j.palaeo.2018.04.004
[15]	Liu, D., Chen, T., Dai, Z., Papineau, D., Qiu, X., Wang, H., Benzerara, K., 2024. A Non-Classical Crystallization Mechanism of Microbially-Induced Disordered Dolomite.Geochimica et Cosmochimica Acta381, 198-209. https://doi.org/10.1016/j.gca.2024.05.005
[16]	Liu, D., Fan, Q., Papineau, D., Yu, N., Chu, Y., Wang, H., Qiu, X., Wang, X., 2020. Precipitation of Protodolomite Facilitated by Sulfate-Reducing Bacteria: The Role of Capsule Extracellular Polymeric Substances.Chemical Geology533, 119415. https://doi.org/10.1016/j.chemgeo.2019.119415
[17]	Liu, D., Yu, N., Papineau, D., Fan, Q., Wang, H., Qiu, X., She, Z., Luo, G., 2019. The Catalytic Role of Planktonic Aerobic Heterotrophic Bacteria in Protodolomite Formation: Results from Lake Jibuhulangtu Nuur, Inner Mongolia, China.Geochimica et Cosmochimica Acta263, 31-49. https://doi.org/10.1016/j.gca.2019.07.056
[18]	Liu, Y., Wang, H., Zhang, J., Ye, Y., Wang, X., Zhang, S., 2025. Uncover the Diagenetic Water Condition and Dolomite Formation in the Songliao Basin 91 Ma.Journal of Earth Science36 (3), 1109-1128. https://doi.org/10.1007/s12583-024-0079-z
[19]	Liu, Z., Yang, S., Bai, Y., Xiu, J., Yan, H., Huang, J., Wang, L., Zhang, H., Liu, Y., 2011. The Alteration of Cell Membrane of Sulfate Reducing Bacteria in the Presence of Mn(Ⅱ) and Cd(Ⅱ).Minerals Engineering24 (8), 839-844. https://doi.org/10.1016/j.mineng.2011.03.005
[20]	Lu, Y., Yang, X., Lin, Z., Sun, X., Yang, Y., Peckmann, J., 2021. Reducing Microenvironments Promote Incorporation of Magnesium Ions into Authigenic Carbonate Forming at Methane Seeps: Constraints for Dolomite Formation.Sedimentology68 (7), 2945-2964. https://doi.org/10.1111/sed.12919
[21]	Lumsden, D.N., Lloyd, R.V., 1984. Mn(Ⅱ) Partitioning Between Calcium and Magnesium Sites in Studies of Dolomite Origin.Geochimica et Cosmochimica Acta48 (9), 1861-1865. https://doi.org/10.1016/0016-7037(84)90039-5
[22]	Moreira, N.F., Walter, L.M., Vasconcelos, C., McKenzie, J.A., McCall, P.J., 2004. Role of Sulfide Oxidation in Dolomitization: Sediment and Pore-Water Geochemistry of a Modern Hypersaline Lagoon System.Geology32 (8), 701. https://doi.org/10.1130/G20353.1
[23]	Ning, M., Yang, F., Ma, H., Lang, X., Shen, B., 2021. Precipitation of Marinoan Cap Carbonate from Mn-Enriched Seawater.Earth-Science Reviews218, 103666. https://doi.org/10.1016/j.earscirev.2021.103666
[24]	Petrash, D.A., Lalonde, S.V., González-Arismendi, G., Gordon, R.A., Méndez, J.A., Gingras, M.K., Konhauser, K.O., 2015. Can Mn-S Redox Cycling Drive Sedimentary Dolomite Formation? A Hypothesis.Chemical Geology404, 27-40. https://doi.org/10.1016/j.chemgeo.2015.03.017
[25]	Qiu, X., Wang, H., Yao, Y., Duan, Y., 2017. High Salinity Facilitates Dolomite Precipitation Mediated byHaloferax volcaniiDS52.Earth and Planetary Science Letters472, 197-205. https://doi.org/10.1016/j.epsl.2017.05.018
[26]	Raudsepp, M.J., Wilson, S., Zeyen, N., Arizaleta, M.L., Power, I.M., 2024. Magnesite Everywhere: Formation of Carbonates in the Alkaline Lakes and Playas of the Cariboo Plateau, British Columbia, Canada.Chemical Geology648, 121951. https://doi.org/10.1016/j.chemgeo.2024.121951
[27]	Roberts, J.A., 2024. The Problem with Dolomite.Nature Geoscience17 (8), 716. https://doi.org/10.1038/s41561-024-01490-6
[28]	Sun, F., Hu, W., Wang, X., Hu, Z., Wu, H., Guo, Y., Wei, G., 2024. Methanogen-Mediated Dolomite Precipitation in an Early Permian Lake in Northwestern China.Geological Society of America Bulletin136 (7-8), 2637-2645. https://doi.org/10.1130/B37156.1
[29]	Toyama, K., Terakado, Y., 2017. An Experimental Study of Na, Mn, Sr and Ba Partitioning Between Dolomite and MgCl2Solution at 130 and 200 ℃.Geochemical Journal51 (6), 199-204. https://doi.org/10.2343/geochemj.2.0444
[30]	Vasconcelos, C., McKenzie, J.A., Bernasconi, S., Grujic, D., Tiens, A.J., 1995. Microbial Mediation as a Possible Mechanism for Natural Dolomite Formation at Low Temperatures.Nature377 (6546), 220-222. https://doi.org/10.1038/377220a0
[31]	Wang, J., Zhao, Y., Li, D., Qi, P., Gao, X., Guo, N., Meng, R., Tucker, M.E., Yan, H., Han, Z., 2022. Extreme Halophilic Bacteria Promote the Surface Dolomitization of Calcite Crystals in Solutions with Various Magnesium Concentrations.Chemical Geology606, 120998. https://doi.org/10.1016/j.chemgeo.2022.120998
[32]	Wright, M.H., Farooqui, S.M., White, A.R., Greene, A.C., 2016. Production of Manganese Oxide Nanoparticles by Shewanella Species.Applied and Environmental Microbiology82 (17), 5402-5409. https://doi.org/10.1128/AEM.00663-16
[33]	You, X., Sun, S., Zhu, J., Li, Q., Hu, W., Dong, H., 2013. Microbially Mediated Dolomite in Cambrian Stromatolites from the Tarim Basin, North-West China: Implications for the Role of Organic Substrate on Dolomite Precipitation.Terra Nova25 (5), 387-395. https://doi.org/10.1111/ter.12048
[34]	Yu, W., Polgári, M., Gyollai, I., Fintor, K., Szabó, M., Kovács, I., Fekete, J., Du, Y., Zhou, Q., 2019. Microbial Metallogenesis of Cryogenian Manganese Ore Deposits in South China.Precambrian Research322, 122-135. https://doi.org/10.1016/j.precamres.2019.01.004
[35]	Zhang, F., Xu, H., Konishi, H., Kemp, J.M., Roden, E.E., Shen, Z., 2012a. Dissolved Sulfide-Catalyzed Precipitation of Disordered Dolomite: Implications for the Formation Mechanism of Sedimentary Dolomite.Geochimica et Cosmochimica Acta97, 148-165. https://doi.org/10.1016/j.gca.2012.09.008
[36]	Zhang, F., Xu, H., Konishi, H., Shelobolina, E.S., Roden, E., 2012b. Polysaccharide-Catalyzed Nucleationand Growth of Disordered Dolomite: A Potential Precursor of Sedimentary Dolomite.American Mineralogist97 (4), 556-567. https://doi.org/10.2138/am.2012.3979
[37]	Zhao, Y.-Y., Wei, X.-Y., Gao, X., Guo, N., Li, J., Hu, K.-M., Han, C., Wang, Q.-Y., Han, Z.-Z., 2025. A Comparison of the Proto-Dolomite Induced by Cyanobacteria and Halophilic Bacteria: Implications for Dolomite-Inducing Microbe Identification.Journal of Palaeogeography14 (1), 277-290. https://doi.org/10.1016/j.jop.2024.09.002
[38]	Zheng, W., Liu, D., Yang, S., Fan, Q., Papineau, D., Wang, H., Qiu, X., 2021. Transformation of Protodolomite to Dolomite Proceeds under Dry-Heating Conditions.Earth and Planetary Science Letters576, 117249. https://doi.org/10.1016/j.epsl.2021.117249
[39]	Zhou, H., Fu, C., 2020. Manganese-Oxidizing Microbes and Biogenic Manganese Oxides: Characterization, Mn(Ⅱ) Oxidation Mechanism and Environmental Relevance.Reviews in Environmental Science and Biotechnology19 (3), 489-507. https://doi.org/10.1007/s11157-020-09541-1
[40]	Zhuang, D., Yan, H., Tucker, M. E., Zhao, H., Han, Z., Zhao, Y., Sun, B., Li, D., Pan, J., Zhao, Y., Meng, R., Shan, G., Zhang, X.,&Tang, R., 2018. Calcite Precipitation Induced byBacillus cereusMRR2 Cultured at Different Ca2+Concentrations: Further Insights into Biotic and Abiotic Calcite.Chemical Geology, 500, 64-87. https://doi.org/10.1016/j.chemgeo.2018.09.018

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锰离子对微生物诱导白云石沉淀的影响

doi: 10.3799/dqkx.2026.179

作者简介:
蔡新宇(2000-),环境科学与工程硕士研究生,主要从事微生物-矿物相互作用的研究。ORCID:0009-0005-6771-0431,E-mail:414446894@qq.com

通讯作者:
王红梅,ORCID:0000-0001-7621-7810,E-mail:wanghmei04@163.com。研究工作受到国家自然基金委重点基金项目资助(42330201)

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Influence of Mn²⁺ on Microbe-Induced Protodolomite Formation

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留言板

锰离子对微生物诱导白云石沉淀的影响

doi: 10.3799/dqkx.2026.179

作者简介: 蔡新宇(2000-),环境科学与工程硕士研究生,主要从事微生物-矿物相互作用的研究。ORCID:0009-0005-6771-0431,E-mail:414446894@qq.com

通讯作者: 王红梅,ORCID:0000-0001-7621-7810,E-mail:wanghmei04@163.com。研究工作受到国家自然基金委重点基金项目资助(42330201)

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出版历程

Influence of Mn2+ on Microbe-Induced Protodolomite Formation

计量

出版历程

目录

作者简介:
蔡新宇(2000-),环境科学与工程硕士研究生,主要从事微生物-矿物相互作用的研究。ORCID:0009-0005-6771-0431,E-mail:414446894@qq.com

通讯作者:
王红梅,ORCID:0000-0001-7621-7810,E-mail:wanghmei04@163.com。研究工作受到国家自然基金委重点基金项目资助(42330201)

Influence of Mn²⁺ on Microbe-Induced Protodolomite Formation