MICROCOSM STUDY OF BENZENE DEGRADATION ON NITRATE REDUCING CONDITIONS
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摘要: 探讨了厌氧微环境中苯的生物降解.接种物来自北京郊区的稻田土.结果表明, 在富集培养和转移培养微环境中, 苯和甲苯的降解与硝酸盐还原作用同时发生.甲苯比苯更易降解, 甲苯的存在促进了苯的降解.这是一例反硝化条件下苯能被生物降解的研究Abstract: This paper discusses the biodegradation of benzene in anaerobic microcosms. The incovula were from the suburban rice soil. The results indicate that benzene and toluene degradation occurred concomitantly with nitrate reduction in enrichment and transfer cultures. Toluene was degraded more readily than benzene, and benzene degradation was enhanced by the presence of toluene. This is an instance of benzene degradation on nitrate reducing conditions.
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Key words:
- biodegradation /
- nitrate reduction /
- benzene /
- microcosm
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表 1 微环境组成
Table 1. Constitution of microcosms
表 2 微环境中硝酸根的消耗量
Table 2. Reduction of nitrate in microcosms
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[1] Wilson J T, McNabb J F, Wilson B H, et al. Biotransformation of selected organic pollutants in ground water[J]. Dev Ind Microbiol, 1983, 24: 225~23. [2] Song HG, Bartha R. Bioremediation potential of terrestrial fuel spills[J]. Applied Environ Microbiol, 1990, 56: 652~656. doi: 10.1128/aem.56.3.652-656.1990 [3] Lovley D R, Woodward J C, Chapelle F H. Rapid anaerobic benzene oxidation with a variety of chelated Fe(Ⅲ) forms[J]. Applied Environ Microbiol, 1996, 62: 288~291. doi: 10.1128/aem.62.1.288-291.1996 [4] Anderson R T, Rooney-Varga J N, Gaw C V, et al. Anaerobic benzene oxidation in the Fe(Ⅲ)reduction zone of petroleum-contaminated aquifers[J]. Environ Sci Technol, 1988, 32: 1222~1229. [5] Edwards E A, Grbic-Galic D. Complete mineralization of benzene by aquifer microorganisms under strictly anaerobic conditions[J]. Applied Environ Microbiol, 1992, 58: 2663~2666. doi: 10.1128/aem.58.8.2663-2666.1992 [6] Weiner J M, Lovley D R. Anaerobic benzene degradation in petroleum-contaminated aquifer sediments after inoculation with a benzene-oxidizing enrichment[J]. Applied Environ Microbiol, 1998, 64: 775~778. doi: 10.1128/AEM.64.2.775-778.1998 [7] Edwards E A, Grbic-Galic D. Anaerobic degradation of toluene and o-xylene by a methanogenic consortium[J]. Applied Environ Microbiol, 1994, 60: 313~322. doi: 10.1128/aem.60.1.313-322.1994 [8] Weiner J M, Lovley D R. Rapid benzene degradation in methanogenic sediments from a petroleum-contaminated aquifer[J]. Applied Environ Microbiol, 1998, 64: 1937~1939. doi: 10.1128/AEM.64.5.1937-1939.1998 [9] Kuhn E P, Zeyer J, Eicher P, et al. Anaerobic degradation of alkylated benzenes in denitrifying laboratory aquifer columns[J]. Applied Environ Microbiol, 1988, 54: 490~ 496. doi: 10.1128/aem.54.2.490-496.1988 [10] Lovley D R. Potential for anaerobic bioremediation of BTEX in petroleum-contaminate aquifers[J]. Journal of Industrial Microbiology& Biotechnology, 1997, 18: 75~81. [11] Alvarez P J J, Vogel T M. Degradation of BTEX and their aerobic metabolites by indigenous microorganisms under nitrate reducing conditions[J]. Wat Sci Technol, 1995, 31: 15~28. [12] Major D W, Mayfield C L, Barker J F. Biotransformation of benzene by denitrification in aquifer sand[J]. Ground Water, 1988, 26: 8~14. doi: 10.1111/j.1745-6584.1988.tb00362.x [13] Burland S M, Edwards E A. Anaerobic benzene biodegradation linked nitrate reduction[J]. Applied Environ Microbiol, 1999, 65: 529~533. doi: 10.1128/AEM.65.2.529-533.1999 [14] 吴玉成, 钟佐, 张建立. 反硝化条件下微生物降解地下水中的苯和甲苯[J]. 中国环境科学, 1999, 19: 505~509. doi: 10.3321/j.issn:1000-6923.1999.06.007 [15] Alvarez P J J, Timothy M V. Substrate interactions of benzene, toluene, and para-xylene during microbial degradation by pure cultures and mixed culture aquifer slurries[J]. Applied Environ Microbiol, 1991, 57: 2981~2985. doi: 10.1128/aem.57.10.2981-2985.1991